产品名称
Anti-Myosin Light Chain Antibody, phospho-specific (Ser19), Chemicon®, from rabbit
biological source
rabbit
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse, human
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
phosphorylation (pSer19)
Quality Level
Gene Information
human ... MYBPHL(343263)
Application
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytoskeleton
Cytoskeleton
This Anti-Myosin Light Chain Antibody, phospho-specific (Ser19) is validated for use in ELISA, WB for the detection of Myosin Light Chain.
Western blot: 1:1,000-1:5,000 using ECL.
ELISA: 1:120,000-1:135,000
Immunohistochemistry: 2.5 μg/mL
ELISA: 1:120,000-1:135,000
Immunohistochemistry: 2.5 μg/mL
Biochem/physiol Actions
Myosin, human, smooth and non-muscle regulatory light chain, phosphoSer19. By Western blot the antibody recognizes the ~20 kDa phosphorylated protein. The immunogen sequence differs from that of the sarcomeric/cardiac form of myosin regulatory light chain. The antibody recognizes monophosphorylated (pSer19) and diphosporylated (pThr18-pSer19) forms of the protein. Reactivity to non-phosphorylated human myosin, smooth and non-muscle regulartory light chain is minimal (less than 1%) by ELISA.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Immunogen
Epitope: phospho-specific (Ser19)
Synthetic phosphopeptide from human myosin, smooth and non-muscle regulatory light chain.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Affinity purified immunoglobulin. Liquid in 0.02M Potassium phosphate, pH 7.2 with 0.15 M Sodium chloride and 0.01% sodium azide.
Preparation Note
Maintain at -20°C in undiluted for up to 6 months from date of receipt. Avoid repeated freeze/thaw cycles. Do not store in a self-defrosting freezer.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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