Anti-Smac/Diablo Antibody

The inhibitor of apoptosis (IAP) proteins regulate programmed cell death by inhibiting members of the caspase family of enzymes. A novel mammalian protein that binds to IAPs and neutralizes their inhibitory effect on caspases has been designated Smac/DIABLO. This is a mitochondrial protein that is released along with cytochrome c during apoptosis and activates the cytochrome c/Apaf-1/caspase-9 pathway. Analysis of the structural basis of Smac/DIABLO reveals that the N-terminal amino acids are required for binding of Smac/DIABLO to IAPs and activation of caspases. Smac/DIABLO is expressed in a variety of human and mouse tissues.

Peptide corresponding to aa 225-239 (EERAESEQEAYLRED) of human Smac/DIABLO.

This Anti-Smac/Diablo Antibody is validated for use in WB for the detection of Smac/Diablo.

Western blot: 1:500 to 1:1000

Human heart tissue lysate can be used as a positive control.

Optimal working dilutions must be determined by end user.

Detects Smac/DIABLO in an approximately 25 kDa band by western blot.

Format: Purified

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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