Anti-Connexin 43 Antibody, phospho-specific (Ser368)

Epitope: phospho-specific (Ser368)

KLH-conjugated, synthetic phospho-peptide corresponding to residues surrounding Ser368 of human Connexin 43.

Western blotting 1:1,000 (for best results, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight)

Optimal working dilutions must be determined by the end user.

Research Sub Category
Adhesion (CAMs)

This Anti-Connexin 43 Antibody, phospho-specific (Ser368) is validated for use in WB for the detection of Connexin 43.

Research Category
Cell Structure

Recognizes Ser368 phosphorylated Connexin 43. Connexin 43 (Cx43) is a member of the large family of gap junction proteins. Connexins assemble as a hexamer and are transported to the plasma membrane to create a hemichannel that can associate with hemichannels on nearby cells to create cell-to-cell channels. Clusters of these channels assemble to make gap junctions. Gap-junction communication is important in development and regulation of cell growth.

Protein A and peptide affinity purified immunoglobulin. Liquid in 10 mM sodium HEPES, pH 7.5, 150 mM NaCl, 100 μg/mL BSA, with 50% glycerol.

Maintain at -20ºC for 12 months after date of receipt. Do not aliquot.

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