AB4332
Anti-acetyl-MVH (Lys405) Antibody
from rabbit, purified by affinity chromatography
别名:
Probable ATP-dependent RNA helicase DDX4, DEAD box protein 4, Mvh, Vasa homolog
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
polyclonal
Species reactivity:
pig, human, mouse
Application:
DB, ICC, IP, WB, inhibition assay
Citations:
-
biological source
rabbit
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
pig, human, mouse
species reactivity (predicted by homology)
porcine (based on 100% sequence homology), rat (based on 100% sequence homology)
technique(s)
dot blot: suitable, immunocytochemistry: suitable, immunoprecipitation (IP): suitable, inhibition assay: suitable (peptide), western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
acetylation (Lys405)
Quality Level
Gene Information
mouse ... Ddx4(13206)
General description
76 kDa calculated
MVH (also known as DDX4 or Vasa) is a member of the DEAD family of proteins and is a probable ATP-dependent RNA helicase. MVH is mostly localized in testicular germ cells in the cytoplasm of spermatocytes. MVH is a high-copy extragenic suppressor gene. MVH is shown to be significantly decreased in spermatozoa of men that are infertile and may have an important role in male fertility and in stem cell development.
Immunogen
Epitope: Acetylated Lys405
KLH-conjugated linear peptide corresponding to mouse MVH acetylated at Lys405.
Application
Anti-acetyl-MVH (Lys405) Antibody is a Rabbit Polyclonal Antibody for detection of acetyl-MVH (Lys405) also known as Probable ATP-dependent RNA helicase DDX4, DEAD box protein & has been validated in DB, WB, ICC, PIA & IP.
Research Category
Stem Cell Research
Epigenetics & Nuclear Function
Stem Cell Research
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Cell Cycle, DNA Replication & Repair
Western Blot/Immunoprecipitation Analysis: A representative lot detected Flag-tagged acetyl-MVH (Lys405) in the presence of Myc-HAT/p46.
Immunocytochemistry Analysis: A representative lot from an independent laboratory detected acetyl-MVH (Lys405) in mouse germ cells (I. Nagamori, Department of Pharmacology, University of California, Irvine).
Immunocytochemistry Analysis: A representative lot from an independent laboratory detected acetyl-MVH (Lys405) in mouse germ cells (I. Nagamori, Department of Pharmacology, University of California, Irvine).
Biochem/physiol Actions
This antibody specifically recognizes wild type MVH acetylated at Lys405, but not the Lys405 mutant.
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Non-acetylated and acetylated MVH (Lys405)
Non-acetylated and acetylated MVH (Lys405)
Evaluated by Dot Blot/Peptide Inhibition Assay in non-acetylated and acetylated MVH (Lys405).
Dot Blot/Peptide Inhibition Analysis: A 1:1,000 dilution of this antibody detected acetyl-MVH (Lys405) peptide.
Dot Blot/Peptide Inhibition Analysis: A 1:1,000 dilution of this antibody detected acetyl-MVH (Lys405) peptide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
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