产品名称
Anti-Calcium Channel Antibody, Voltage Gated α 1A, Chemicon®, from rabbit
biological source
rabbit
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
rat, mouse
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... CACNA1A(773)
Analysis Note
Control
CONTROL ANTIGEN: Included free of charge with the antibody is 20 μg of control antigen (lyophilized powder in phosphate buffered saline, pH 7.4, containing 5% sucrose and 0.025% sodium azide). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 10 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
CONTROL ANTIGEN: Included free of charge with the antibody is 20 μg of control antigen (lyophilized powder in phosphate buffered saline, pH 7.4, containing 5% sucrose and 0.025% sodium azide). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 10 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Application
Anti-Calcium Channel Antibody, Voltage Gated α 1A is an antibody against Calcium Channel for use in IC, IP & WB.
In preparing full-length protein samples for Western blot or immunoprecipitation, the procedures should be performed at 4°C, with the following protease inhibitor mixture: pepstatin A (1 μg/mL), leupeptin (1 μg/mL), aprotinin (1 μg/mL), Pefabloc SC (0.2 mM), benzamidine (0.1 mg/mL), and calpain inhibitors I and II (8 μg/mL each)
Immunoprecipitation: 3 μg/mL
Immunocytochemistry: 5 μg/mL (1:60). Recommend subsequent staining with a labeled
second antibody.
Western blot: 1.5 μg/mL (1:200) using ECL
Dilutions should be made using a carrier protein such as BSA (1-3%)
Optimal working dilutions must be determined by the end user.
Immunoprecipitation: 3 μg/mL
Immunocytochemistry: 5 μg/mL (1:60). Recommend subsequent staining with a labeled
second antibody.
Western blot: 1.5 μg/mL (1:200) using ECL
Dilutions should be made using a carrier protein such as BSA (1-3%)
Optimal working dilutions must be determined by the end user.
Biochem/physiol Actions
Recognizes Cav2.1 (alpha 1A subunit, P/Q-type of voltage-gated calcium channel, Cacna1a). Does not cross react with any other calcium channel proteins tested so far.
Immunogen
Purified peptide CNA1 from 865-881 of alpha 1A subunit of rat brain voltage-gated calcium channel (Starr et al. 1991) (Accession P54282) containing N-terminal lysine and tyrosine, conjugated to KLH with gluteraldehyde (Westenbroek et al. 1995).
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Preparation Note
Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution add glycerol (ASC gade or better at a ratio of 1:1 and maintain at -20°C for up to 6 months. Avoid repeated freeze/thaw cycles.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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hcodes
pcodes
Hazard Classifications
Aquatic Chronic 3
存储类别
11 - Combustible Solids
wgk
WGK 3
Christoph Limbach et al.
Proceedings of the National Academy of Sciences of the United States of America, 108(31), E392-E401 (2011-06-30)
The protein machinery of neurotransmitter exocytosis requires efficient orchestration in space and time, for speed and precision of neurotransmission and also for synaptic ontogeny and plasticity. However, its spatial organization in situ is virtually unknown. Aczonin/Piccolo is a putative organizer
Arn M J M van den Maagdenberg et al.
Neuron, 41(5), 701-710 (2004-03-09)
Migraine is a common, disabling, multifactorial, episodic neurovascular disorder of unknown etiology. Familial hemiplegic migraine type 1 (FHM-1) is a Mendelian subtype of migraine with aura that is caused by missense mutations in the CACNA1A gene that encodes the alpha(1)
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