AB5338P
Anti-Cocaine and Amphetamine Related Transcript Antibody
Chemicon®, from rabbit
别名:
CART/CARP
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ELISA
immunohistochemistry
western blot
immunohistochemistry
western blot
Species reactivity:
mouse, rat
Citations:
4
Technique(s):
ELISA: suitable
immunohistochemistry: suitable
western blot: suitable
immunohistochemistry: suitable
western blot: suitable
Uniprot accession no.:
产品名称
Anti-Cocaine and Amphetamine Related Transcript Antibody, Chemicon®, from rabbit
Quality Level
biological source
rabbit
conjugate
unconjugated
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse, rat
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunohistochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
mouse ... Cartpt(27220)
rat ... Cartpt(29131)
Application
Research Category
Neuroscience
Neuroscience
Research Sub Category
CNS Control of Metabolism
Reward & Addiction
CNS Control of Metabolism
Reward & Addiction
This Anti-Cocaine & Amphetamine Related Transcript Antibody is validated for use in ELISA, WB, IH for the detection of Cocaine & Amphetamine Related Transcript.
Western blotting: 1-10 μg/mL using ECL.
Immunohistochemistry: 5-10 μg/mL It has been reported that the antibody will recognize CART in neurons (cell bodies) from the rat hypothalamic arcuate nucleus.
ELISA: 1:10,000-1:50,000 using 50-100 ng control peptide (Catalog Number AG228) per well.
Optimal working dilutions must be determined by the end user.
Immunohistochemistry: 5-10 μg/mL It has been reported that the antibody will recognize CART in neurons (cell bodies) from the rat hypothalamic arcuate nucleus.
ELISA: 1:10,000-1:50,000 using 50-100 ng control peptide (Catalog Number AG228) per well.
Optimal working dilutions must be determined by the end user.
Biochem/physiol Actions
Recognizes Cocaine- and Amphetamine Related Transcript (CART). CART has been found to control satiety modulating the actions of two key regulators of food intake, leptin and NPY. Starvation decreases CART levels in the arcuate nucleus (Kristensen et al. 1998). Obese animals have virtually no CART. CART inhibits both normal and starvation-induced feeding, and completely inhibits NPY-induced feeding. Immunoneutralization of CART by administration of anti-CART resulted into higher food intake suggesting that CART is an endogenous regulator of food intake.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Immunogen
Mouse/rat CART peptide (55-102).
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Affinity purified immunoglobulin. Liquid in PBS containing 0.1% BSA. No preservative.
Preparation Note
Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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