Anti-Cocaine and Amphetamine Related Transcript Antibody

Mouse/rat CART peptide (55-102).

Detect Cocaine & Amphetamine Related Transcript using this Anti-Cocaine & Amphetamine Related Transcript Antibody validated for use in ELISA, WB, IH.

Research Category
Neuroscience

Research Sub Category
CNS Control of Metabolism

Reward & Addiction

Western blotting: 1-10 μg/mL using ECL.

Immunohistochemistry: It has been reported that the antibody will recognize CART in neurons (cell bodies) from the rat hypothalamic arcuate nucleus.

ELISA: 1:10,000-1:50,000 using 50-100 ng control peptide per well.

Optimal working dilutions must be determined by the end user.

Recognizes Cocaine and Amphetamine Related Transcript (CART). CART has been found to control satiety modulating the actions of two key regulators of food intake, leptin and NPY. Starvation decreases CART levels in the arcuate nucleus (Kristensen et al. 1998). Obese animals have virtually no CART. CART inhibits both normal and starvation-induced feeding, and completely inhibits NPY-induced feeding. Immunoneutralization of CART by administration of anti-CART resulted into higher food intake suggesting that CART is an endogenous regulator of food intake.

Affinity purified immunoglobulin. Liquid in PBS with 0.1% BSA

Maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.