GAP-43, a common marker of differentiating neurons, is expressed at elevated levels by developing or regenerating neurons during axon growth. While GAP-43 is an integral membrane protein associated with the cytoplasmic surface of axonal growth cones (and synapses), it is absent from dendritic growth cones. In adult brain, GAP-43 is found in high concentration in presynaptic areas where memory formation is thought to occur, such as frontal cortex, limbic system and hippocampus. Phosphorylation of GAP-43 by PKC (at Ser41 on human, mouse, rat, and bovine GAP-43 or Ser42 on chicken and Xenopus GAP-43) is specifically correlated with certain forms of synaptic plasticity.

Epitope: phosphoSer 41

Synthetic peptide corresponding to amino acids surrounding the phosphoSer41 of rat GAP-43. This is the site phosphorylated by protein kinase C.

Anti-Growth Associated Protein 43 Antibody, phosphoSer 41 is an antibody against Growth Associated Protein 43 for use in WB.

Research Category
Neuroscience

Research Sub Category
Neuroregenerative Medicine

Signaling Neuroscience

Western blot: 1:1,000 (43kDa band).

Optimal working dilutions must be determined by the end user.

GAP-43 phosphoSer41. The labeling of the GAP-43 protein (~43kDa) in Western blots of rat brain is blocked by the phosphopeptide but not by the dephosphopeptide. In some tissues the antibody may recognize a lower molecular weight band. This band is also detected by a pan GAP-43 antibody.

Affinity purified immunoglobulin in 10 mM HEPES (pH 7.5), 150 mM NaCl with 100 μg/mL BSA and 50% glycerol.

Maintain at -20°C in undiluted for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. Do not store in a self defrosting freezer.

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