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Merck
CN

AB5509

抗阿片受体µ抗体

Chemicon®, from guinea pig

别名:

MOR-1

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ICC, IHC
Citations:
9
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biological source

guinea pig

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

primate, rat, mouse

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Quality Level

Gene Information

human ... OPRM1(4988)

Immunogen

来自大鼠mu型阿片受体C末端的合成肽(目录号AG375),对应于大鼠mu阿片受体的残基384-398。

Application

免疫组化:1:50-500 参见方案。

免疫细胞化学:1:50-500 参见方案。

最佳工作稀释度必须由最终用户进行确定。

IHC方案: 雄性Sprague-Dawley大鼠(b.wt.100-150g)用戊巴比妥钠麻醉,并通过升主动脉灌注:1)50 mL不含Ca2+的台氏液,然后是2)多聚甲醛-苦味酸固定剂和3)10%蔗糖的PBS溶液作为冷冻保护剂。快速解剖组织,并在含有10%蔗糖的0.1 M磷酸盐缓冲液(pH值7.4)中保存过夜。将载玻片固定的组织切片与封闭缓冲液在室温下培养1小时。将一抗在封闭缓冲液中稀释至适当的工作稀释度。除去封闭缓冲液,然后将载玻片与AB5509在2-8°C下培养18-24小时。在PBS中冲洗3次后,将切片与Cy3偶联的二抗在室温下培养60分钟。固定在含有0.1%对苯二胺的PBS和甘油(1:3)的混合物中后,用Nikon Microphot-SA落射荧光显微镜检查切片。

ICC方案: 对Mu型阿片受体转染的细胞进行间接免疫荧光。除去培养基,并用无血清培养基轻轻洗涤细胞3次。除去培养基,并用无血清培养基轻轻洗涤细胞3次。固定后,如上所述对细胞进行间接免疫荧光处理。
抗阿片受体μ抗体-AB5509是用于IC,IH的针对阿片受体的抗体。
研究子类别
神经炎症&疼痛
研究类别
神经科学

Biochem/physiol Actions

Mu型阿片受体

Physical form

含50%甘油的PBS;浓度1.0 mg/ml

Preparation Note

自收到之日起,在-20° C以未稀释的等分试样可保存 6 个月。应避免反复冻/融循环。

Other Notes

替代品:AB1774

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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存储类别

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Autism is characterized by atypical social communication and stereotyped behaviors. Mutations in the gene encoding the synaptic scaffolding protein SHANK3 are detected in 1-2% of patients with autism and intellectual disability, but the mechanisms underpinning the symptoms remain largely unknown.
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Opioid analgesic tolerance, a root cause of opioid overdose and misuse, can develop through an associative learning. Despite intensive research, the locus and central pathway subserving the associative opioid analgesic tolerance (AOAT) remains unclear. Using a combination of chemo/optogenetic manipulation
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Dopamine (DA) neurons derived from human embryonic stem cells (hESCs) are a promising unlimited source of cells for cell replacement therapy in Parkinson's disease (PD). A number of studies have demonstrated functionality of DA neurons originating from hESCs when grafted
D Gagnon et al.
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The loss of nigrostriatal dopamine neurons in Parkinson's disease induces a reduction in the number of dendritic spines on medium spiny neurons (MSNs) of the striatum expressing D1 or D2 dopamine receptor. Consequences on MSNs expressing both receptors (D1/D2 MSNs)

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