特异性
Recognizes Kv1.3, extracellular (Kcna3).
免疫原
Epitope: extracellular domain
Highly purified peptide corresponding to residues 263-275 of human Kv1.3 (Accession P22001).
应用
Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters
This Anti-Potassium Channel Kv1.3 Antibody, extracellular domain is validated for use in FC, WB for the detection of Potassium Channel Kv1.3.
Western blotting: 1:200-1:500 using ECL on rat brain membranes.
Indirect flow cytometry: 0.5-5 μg of antibody per 1x10e6cells.
Dilutions should be made using a carrier protein such as BSA (1-3%).
Optimal working dilutions must be determined by the end user.
外形
Affinity purified immunoglobulin. Lyophilized from PBS, pH 7.4, containing 1% BSA and 0.05% sodium azide. Reconstitute with 200 μL of sterile distilled water. Centrifuge antibody preparation before use (10,000 x g for 5 min).
储存及稳定性
Maintain lyophilized material at -20°C for up to 6 months after date of receipt. After reconstitution maintain antibody and control peptide at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
分析说明
Control
Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). For negative control, preincubate 5 μg of purified peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.