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Merck
CN

AB5622

Anti-MAP2 Antibody

CHEMICON®, rabbit polyclonal

别名:

抗 MAP2 抗体

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关于此项目

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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产品名称

抗微管相关蛋白2(MAP2)抗体, Chemicon®, from rabbit

biological source

rabbit

antibody form

saturated ammonium sulfate (SAS) precipitated

antibody product type

primary antibodies

clone

polyclonal

species reactivity

rat, mouse, human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

target post-translational modification

unmodified

Quality Level

Gene Information

human ... MAP2(4133)
mouse ... Map2(17756)
rat ... Map2(25595)

Analysis Note

对照
人脑组织或人胶质母细胞瘤T98G细胞
免疫组化(石蜡)分析:
正常小脑上的MAP2(目录号AB5622)染色。 用pH 6.0的柠檬酸盐预处理的组织。使用带有HRP-DAB的IHC-Select Detection将这批抗体稀释至1:500。 免疫反应性表现为微管上的纤维样染色(棕色)。
使用TE缓冲液,pH值9.0进行最佳染色,表位修复:人小脑

Application

使用该抗微管相关蛋白2(MAP2)抗体检测微管相关蛋白2(MAP2),该抗体经验证可用于ELISA、IC、IH、IH(P)&WB,产品引用次数超过55次。
免疫细胞化学:
先前批次的1:1,000稀释液用于原代神经元。

免疫组化:
在4%多聚甲醛/PBS固定的组织切片上以1:1,000的稀释度进行。

免疫印迹:
在先前批次上使用1:2,000的稀释液。 识别MAP2A(280-300kDa)和MAP2B(270-280kDa),并在较小程度上识别MAP2C(70kDa)和MAP2D(70-75kDa)。

ELISA:
使用大于或等于先前批次的1:2,000稀释液。

最佳工作稀释度必须由最终用户确定。
研究子类别
神经 & 胶质标志物

神经丝 & 神经元代谢
研究类别
神经科学

Biochem/physiol Actions

抗 MAP2 抗体与其他物种的反应性暂不确定。 抗 MAP2 抗体对微管相关蛋白 2 (MAP2) 具有特异性。该抗体可识别全部MAP2亚型(MAP2A、MAP2B、MAP2C和MAP2D)。它与HMW-MAP2(MAP2A和MAP2B)显示出最大的免疫反应性。

Disclaimer

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

General description

SDS-PAGE上的约280 kDa双峰(MAP2a和MAP2b)和约70 kDa双峰(MAP2c)。
微管相关蛋白2(MAP2)是一种丰富的神经元细胞骨架蛋白,可与微管蛋白结合并稳定微管。(Herzog and Weber, 1978).MAP-2对于神经元形态的发育和维持至关重要(Matus,1991)。在神经元中,MAP2以三种主要亚型出现,即高分子量MAP2a,MAP2b和低分子量MAP2c,它们是由MAP2基因的选择性剪接产生的(Chung et al,1996)。低分子量亚型MAP2c在发育中的大脑中表达,并在大脑成熟过程中被下调,而高分子量MAP2b在发育中和成年大脑中均表达。MAP2a仅在大脑成熟后出现(Tucker,1990)。全部这些形式通过羧基末端附近的结构域与微管结合,该结构域包含31个氨基酸基序的三个或四个相似重复序列(Lewis et al.,1988)。MAP-2与MAP-4和tau蛋白一起属于与微管相关的热稳定蛋白家族。

Immunogen

从大鼠脑中纯化的微管相关蛋白。

Physical form

在含有0.1%叠氮化钠作为防腐剂的PBS的缓冲液中的纯化兔多克隆抗体。
形式:纯化
硫酸铵沉淀

Preparation Note

自收到之日起,在 -20ºC 以未稀释的等分试样可保存 6 个月。避免反复冻/融循环。

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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存储类别

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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Mitochondrial protection attenuates inflammation-induced impairment of neurogenesis in vitro and in vivo.
Voloboueva, LA; Lee, SW; Emery, JF; Palmer, TD; Giffard, RG
The Journal of Neuroscience null
Neil G Harris et al.
Journal of neuropathology and experimental neurology, 69(2), 139-154 (2010-01-20)
We previously reported that pericontusional extracellular chondroitin sulfate proteoglycans (CSPGs) are profoundly reduced for 3 weeks after experimental traumatic brain injury, indicating a potential growth-permissive window for plasticity. Here, we investigate the extracellular environment of sprouting neurons after controlled cortical
Chengrui Nan et al.
International journal of molecular medicine, 36(4), 1057-1062 (2015-08-12)
In the present study, human umbilical cord-derived mesenchymal stem cells (hUMSCs) were investigated for their potential to be induced to differentiate in vitro into neuron-like cells by monosialoteterahexosyl ganglioside (GM1). Mononuclear cells obtained from umbilical cords from women with full-term pregnancies whose babies
Anne M Taylor et al.
Neuron, 66(1), 57-68 (2010-04-20)
The polarized nature of neurons and the size and density of synapses complicates the manipulation and visualization of cell biological processes that control synaptic function. Here we developed a microfluidic local perfusion (microLP) chamber to access and manipulate synaptic regions
Krystyna Bajsarowicz et al.
Journal of neuropathology and experimental neurology, 71(5), 449-466 (2012-04-18)
Brain aggregates (BrnAggs) derived from fetal mouse brains contain mature neurons and glial cells. We determined that BrnAggs are consistently infected with Rocky Mountain Laboratory scrapie strain prions and produce increasing levels of the pathogenic form of the prion protein

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