产品名称
抗微管相关蛋白2(MAP2)抗体, Chemicon®, from rabbit
biological source
rabbit
antibody form
saturated ammonium sulfate (SAS) precipitated
antibody product type
primary antibodies
clone
polyclonal
species reactivity
rat, mouse, human
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
target post-translational modification
unmodified
Quality Level
Gene Information
human ... MAP2(4133)
mouse ... Map2(17756)
rat ... Map2(25595)
Analysis Note
人脑组织或人胶质母细胞瘤T98G细胞
正常小脑上的MAP2(目录号AB5622)染色。 用pH 6.0的柠檬酸盐预处理的组织。使用带有HRP-DAB的IHC-Select Detection将这批抗体稀释至1:500。 免疫反应性表现为微管上的纤维样染色(棕色)。
使用TE缓冲液,pH值9.0进行最佳染色,表位修复:人小脑
Application
先前批次的1:1,000稀释液用于原代神经元。
免疫组化:
在4%多聚甲醛/PBS固定的组织切片上以1:1,000的稀释度进行。
免疫印迹:
在先前批次上使用1:2,000的稀释液。 识别MAP2A(280-300kDa)和MAP2B(270-280kDa),并在较小程度上识别MAP2C(70kDa)和MAP2D(70-75kDa)。
ELISA:
使用大于或等于先前批次的1:2,000稀释液。
最佳工作稀释度必须由最终用户确定。
神经 & 胶质标志物
神经丝 & 神经元代谢
神经科学
Biochem/physiol Actions
Disclaimer
General description
Immunogen
Other Notes
Physical form
Preparation Note
Legal Information
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存储类别
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
相关内容
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