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Merck
CN

AB5970

Anti-Dishevelled-1 Antibody

Chemicon®, from rabbit

别名:

DSH, DVL1, DSH Homolog 1

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关于此项目

UNSPSC代码:
12352203
eCl@ss:
32160702
NACRES:
NA.41

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生物来源

rabbit

质量水平

100

偶联物

unconjugated

抗体形式

affinity purified immunoglobulin

抗体产品类型

primary antibodies

克隆

polyclonal

纯化方式

affinity chromatography

种属反应性

human

制造商/商品名称

Chemicon®

技术

western blot: suitable

输入

sample type hematopoietic stem cell(s)
sample type neural stem cell(s)

NCBI登记号

NM_004421.2
NM_181870.1
NM_182779.3

UniProt登记号

O14640

运输

wet ice

靶向翻译后修饰

unmodified

基因信息

human ... DVL1(1855)

一般描述

85 kDa
DVL1, the human homolog of the Drosophila dishevelled gene (dsh) encodes a cytoplasmic phosphoprotein that regulates cell proliferation. The protein acts as a transducer molecule for developmental processes, including segmentation and neuroblast specification. DVL1 mutation is likely responsible for transformation in neuroblastomas, and the Schwartz-Jampel syndrome and Charcot-Marie-Tooth disease type 2A have been mapped to the same region as DVL1 gene, thus DVL1 maybe important in these diseases as well.

免疫原

Synthetic peptide corresponding to amino acids 557-571 of human dishevelled-1 protein.

应用

Anti-Dishevelled-1 Antibody is an antibody against Dishevelled-1 for use in WB.
Research Category
Stem Cell Research
Research Sub Category
Hematopoietic Stem Cells

Neural Stem Cells
Western blot: 1:500 using ECL. The antibody reacts with a protein of ~85kDa. Dilute antibody in 50 mM PBS, pH 7.4 containing 1.5% sodium chloride and 1% normal animal serum (same species as the host of the secondary antibody).

Optimal working dilutions must be determined by end user.

生化/生理作用

Dishevelled-1 protein (dvl-1). By Western blot the antibody shows no cross reactivity with recombinant dishevelled -2 or dishevelled -3 protein Pre-adsorption of the antibody with the immunogen peptide completely abolishes its reactivity by Western blot against dishevelled -1 protein.

外形

Liquid in PBS, pH 7.4, containing 0.01M sodium azide.

制备说明

Maintain at -20°C in undiluted aliquots (not less than 10 μL) for up to 6 months. Avoid repeated freeze/thaw cycles. The use of high quality antiserum-grade plastic or glass is recommended.

分析说明

Control
SK-N-SH (neuroblastoma) whole cell lysates

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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储存分类代码

10 - Combustible liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

分析证书(COA)

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Micha Nethe et al.
Journal of cell science, 125(Pt 14), 3430-3442 (2012-04-03)
The Rho-GTPase Rac1 promotes actin polymerization and membrane protrusion that mediate initial contact and subsequent maturation of cell-cell junctions. Here we report that Rac1 associates with the ubiquitin-protein ligase neural precursor cell expressed developmentally down-regulated 4 (Nedd4). This interaction requires

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