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Merck
CN

AB5970

Anti-Dishevelled-1 Antibody

Chemicon®, from rabbit

别名:

DSH, DVL1, DSH Homolog 1

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UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
western blot
Species reactivity:
human
Citations:
2
Technique(s):
western blot: suitable
Uniprot accession no.:
O14640

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产品名称

Anti-Dishevelled-1 Antibody, Chemicon®, from rabbit

biological source

rabbit

conjugate

unconjugated

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

western blot: suitable

input

sample type hematopoietic stem cell(s)
sample type neural stem cell(s)

NCBI accession no.

NM_004421.2
NM_181870.1
NM_182779.3

UniProt accession no.

O14640

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... DVL1(1855)

Analysis Note

Control
SK-N-SH (neuroblastoma) whole cell lysates

Application

Anti-Dishevelled-1 Antibody is an antibody against Dishevelled-1 for use in WB.
Research Category
Stem Cell Research
Research Sub Category
Hematopoietic Stem Cells

Neural Stem Cells
Western blot: 1:500 using ECL. The antibody reacts with a protein of ~85kDa. Dilute antibody in 50 mM PBS, pH 7.4 containing 1.5% sodium chloride and 1% normal animal serum (same species as the host of the secondary antibody).

Optimal working dilutions must be determined by end user.

Biochem/physiol Actions

Dishevelled-1 protein (dvl-1). By Western blot the antibody shows no cross reactivity with recombinant dishevelled -2 or dishevelled -3 protein Pre-adsorption of the antibody with the immunogen peptide completely abolishes its reactivity by Western blot against dishevelled -1 protein.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

85 kDa
DVL1, the human homolog of the Drosophila dishevelled gene (dsh) encodes a cytoplasmic phosphoprotein that regulates cell proliferation. The protein acts as a transducer molecule for developmental processes, including segmentation and neuroblast specification. DVL1 mutation is likely responsible for transformation in neuroblastomas, and the Schwartz-Jampel syndrome and Charcot-Marie-Tooth disease type 2A have been mapped to the same region as DVL1 gene, thus DVL1 maybe important in these diseases as well.

Immunogen

Synthetic peptide corresponding to amino acids 557-571 of human dishevelled-1 protein.

Physical form

Liquid in PBS, pH 7.4, containing 0.01M sodium azide.

Preparation Note

Maintain at -20°C in undiluted aliquots (not less than 10 μL) for up to 6 months. Avoid repeated freeze/thaw cycles. The use of high quality antiserum-grade plastic or glass is recommended.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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存储类别

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Micha Nethe et al.
Journal of cell science, 125(Pt 14), 3430-3442 (2012-04-03)
The Rho-GTPase Rac1 promotes actin polymerization and membrane protrusion that mediate initial contact and subsequent maturation of cell-cell junctions. Here we report that Rac1 associates with the ubiquitin-protein ligase neural precursor cell expressed developmentally down-regulated 4 (Nedd4). This interaction requires

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