产品名称
胞质FMR1相互作用蛋白1抗体, from rabbit, purified by affinity chromatography
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse, human
species reactivity (predicted by homology)
bovine (based on 100% sequence homology), rat (based on 100% sequence homology), primate (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology)
technique(s)
immunocytochemistry: suitable
western blot: suitable
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... SRA1(10011)
Application
研究子类别
细胞骨架信号转导
细胞骨架信号转导
研究类别
细胞结构
信号传导
细胞结构
信号传导
可使用这种经验证可用于WB & IC的胞质FMR1相互作用蛋白1抗体检测胞质FMR1互相作用蛋白1。
蛋白质印迹法(Snap i.d.)分析: 5 µg/mL代表性批次在10 µg HeLa细胞裂解液中检测到RNA聚合酶II。
免疫细胞化学分析:先前批次的1:500稀释液在NIH/3T3、HeLa和A431细胞中检测到胞质FMR1相互作用蛋白1。
免疫细胞化学分析:先前批次的1:500稀释液在NIH/3T3、HeLa和A431细胞中检测到胞质FMR1相互作用蛋白1。
Analysis Note
对照
HeLa细胞裂解液
HeLa细胞裂解液
通过蛋白质印迹法在HeLa细胞裂解液中进行了评估。
蛋白质印迹分析:1 µg/mL的该抗体在10 µg HeLa细胞裂解液中检测到胞质FMR1相互作用蛋白1。
蛋白质印迹分析:1 µg/mL的该抗体在10 µg HeLa细胞裂解液中检测到胞质FMR1相互作用蛋白1。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
General description
胞质FMR1相互作用蛋白1(CYFIP1)是CYFIP家族的成员。它是CYFIP1-EIF4E-FMR1复合物的组成部分,该复合物是一种与mRNA帽结合的翻译抑制调控复合物。在复合物中,CYFIP1充当其他两种组分之间的衔接器,并上调FMR1的翻译抑制活性。它调控上皮形态发生,结合F-肌动蛋白,是轴突生长、板状伪足、突起和膜皱褶发育和形成的组成部分。通过与RAC1的相互作用,CYFIP1调控WASP家族成员的活性。CYFIP1与Prader-Willi/天使综合征有关,也可能在自闭症中发挥作用。
观测分子量〜154 kDa。 有三种值得注意的同工型,计算分子量分别为51 kDa、94 kDa和145 kDa。在某些裂解液中可能会观测到其他同工型。
Immunogen
KLH偶联线性肽,对应于人胞质FMR1相互作用蛋白1的内部序列(a.a.519-532)。
表位:未知
Other Notes
浓度:请参考批次特异性浓缩物的检验报告。
Physical form
亲和纯化
纯化的兔多克隆抗体,溶于含有0.1 M Tris-甘氨酸(pH 7.4)、150 mM NaCl和0.05%叠氮化钠的缓冲液中。
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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