产品名称
Anti-Fibrinogen Antibody, FITC conjugated, Chemicon®, from sheep
technique(s)
western blot: suitable
immunodiffusion: suitable
immunofluorescence: suitable
biological source
sheep
conjugate
FITC conjugate
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human
manufacturer/tradename
Chemicon®
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... FIBCD1(84929)
Application
Detect Fibrinogend using this Anti-Fibrinogen Antibody, FITC conjugated validated for use in IF, ID & WB.
Research Category
Signaling
Signaling
Research Sub Category
Growth Factors & Receptors
Growth Factors & Receptors
WORKING DILUTION
The final working dilution is dependent on the specific assay conditions. However, in fluorescence microscopy good results can be expected with dilutions in the following ranges:
1. Incident light excitation
Direct immunofluorescence: 1/10 - 1/80
Indirect immunofluorescence: 1/20 - 1/400
2. Transmitted light excitation
Direct immunofluorescence: 1/5 - 1/40
Indirect immunofluorescence: 1/10 - 1/80
The final working dilution is dependent on the specific assay conditions. However, in fluorescence microscopy good results can be expected with dilutions in the following ranges:
1. Incident light excitation
Direct immunofluorescence: 1/10 - 1/80
Indirect immunofluorescence: 1/20 - 1/400
2. Transmitted light excitation
Direct immunofluorescence: 1/5 - 1/40
Indirect immunofluorescence: 1/10 - 1/80
Biochem/physiol Actions
Prior to conjugation, the antibody was specific for fibrinogen when tested against human plasma by Coomassie Blue R stained double immunodiffusion and immunoelectrophoresis. Undesired immune reactivity has been removed by solid phase immunoadsorption.
LABELLING RATIO
Fluorescein:antibody molar ratio: 2.8 - 3.3
Absorbance ratio (A493/A276): 0.78 + 0.05
LABELLING RATIO
Fluorescein:antibody molar ratio: 2.8 - 3.3
Absorbance ratio (A493/A276): 0.78 + 0.05
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Immunogen
Purified fibrinogen derived from pooled human plasma.
Physical form
The antibody is supplied in 20mM Tris, 70mM NaCl, pH 8.0, with 0.1% sodium azide as preservative.
Preparation Note
Store at +2 to +8°C, for up to 6 months, undiluted. Protect from light.
WARNING: This reagent contains 0.1% sodium azide as preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.
WARNING: This reagent contains 0.1% sodium azide as preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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