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AB758

Sigma-Aldrich

Anti-Collagen Type I Antibody

Chemicon®, from goat

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别名:
Anti-CAFYD, Anti-EDSARTH1, Anti-EDSC, Anti-OI1, Anti-OI2, Anti-OI3, Anti-OI4
eCl@ss:
32160702
NACRES:
NA.41

生物来源

goat

质量水平

抗体形式

affinity purified immunoglobulin

antibody product type

primary antibodies

克隆

polyclonal

纯化方式

affinity chromatography

species reactivity

human, bovine

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
dot blot: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

适用性

not suitable for immunohistochemistry (Paraffin)

NCBI登记号

UniProt登记号

运输

wet ice

target post-translational modification

unmodified

Gene Information

human ... COL1A1(1277)

特异性

Recognizes Human type I collagen as demonstrated by ELISA. Less than 10% cross reactivity with collagen types II, III, IV, V, and VI. May show reactivity to type I collagen from other species. AB758 has not been tested with other extracellular matrix proteins (e.g., laminin, fibronectin).

免疫原

Human placental collagen type I isolated by limited pepsin digestion and selective salt precipitation.

应用

Anti-Collagen Type I Antibody is an antibody against Collagen Type I for use in DB, ELISA, IC, IH & WB.
Dot and slot blotting: 1:100-1:500

ELISA: 1:50-1:100

Indirect immunohistochemistry (frozen sections only): 1:10-1:20

Indirect immunocytochemistry: 1:10-1:20

Antibody reacts with human collagen I in reduced westerns with supernatant samples from serum-free cultures of human fibroblasts {LMF, personal communication}.

Not recommended for paraffin tissue sections.

Optimal working dilutions must be determined by the end user.

外形

Affinity Purified immunoglobulin. Prior to purification the antisera was adsorbed against collagen types II, III, IV, V and VI immobilized on Sepharose 4B.. Liquid in 0.5 mL of 100 mM borate buffered saline, pH 8.0, no preservatives.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Sepharose is a trademark of Cytiva

象形图

Health hazard

警示用语:

Danger

危险声明

危险分类

Repr. 1B

储存分类代码

6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

nwg

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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