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Merck
CN

AB758B

Sigma-Aldrich

Anti-Collagen Type I Antibody, biotin conjugated

Chemicon®, from goat

别名:

Anti-CAFYD, Anti-EDSARTH1, Anti-EDSC, Anti-OI1, Anti-OI2, Anti-OI3, Anti-OI4

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关于此项目

UNSPSC代码:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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生物来源

goat

质量水平

偶联物

biotin conjugate

抗体形式

affinity purified immunoglobulin

抗体产品类型

primary antibodies

克隆

polyclonal

种属反应性

bovine, human

制造商/商品名称

Chemicon®

技术

ELISA: suitable
dot blot: suitable
immunohistochemistry: suitable
western blot: suitable

适用性

not suitable for immunohistochemistry (Paraffin)

NCBI登记号

UniProt登记号

运输

wet ice

靶向翻译后修饰

unmodified

基因信息

human ... COL1A1(1277)

免疫原

Human placental collagen type I

应用

Anti-Collagen Type I Antibody, biotin conjugated is an antibody against Collagen Type Id for use in DB, ELISA, WB, IH.
Dot and slot blotting: 1:500-1:1,000

ELISA: 1:1,000-4,000

Indirect immunohistochemistry (frozen sections only): 1:20-1:40

The non-biotinylated version (Cat. # AB758) reacts with human collagen I in reduced westerns with supernatant samples from serum-free cultures of human fibroblasts , and has been cited for use in WB applications (PMID: 20089870)

Not recommended for paraffin tissue sections.

Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Research Sub Category
ECM Proteins

生化/生理作用

Human type I collagen as demonstrated by ELISA. Less than 10% cross reactivity with collagen types II, III, IV, V, and VI. May show reactivity to type I collagen from other species. AB758B has not been tested with other extracellular matrix proteins (e.g., laminin, fibronectin).

外形

Affinity purified immunoglobulin, biotin labeled. Prior to purification the antisera was adsorbed against collagen type II, III, IV, V and VI immobilized on Sepharose 4B.. Liquid in phosphate buffered saline, pH 7.4, containing 0.1% sodium azide.

制备说明

Maintain at 2-8°C in undiluted aliquots for up to 12 months.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Sepharose is a trademark of Cytiva

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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储存分类代码

12 - Non Combustible Liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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Stephen J Atkins et al.
Endocrinology, 156(12), 4731-4740 (2015-09-22)
A relationship between the actions of TSH and IGF-1 was first recognized several decades ago. The close physical and functional associations between their respective receptors (TSHR and IGF-1R) has been described more recently in thyroid epithelium and human orbital fibroblasts
Interleukin-6 production in CD40-engaged fibrocytes in thyroid-associated ophthalmopathy: involvement of Akt and NF-?B.
Gillespie, EF; Raychaudhuri, N; Papageorgiou, KI; Atkins, SJ; Lu, Y; Charara, LK; Mester et al.
Investigative Ophthalmology & Visual Science null

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