AB761
Anti-Collagen Type II (COL2A1) Antibody
CHEMICON®, rabbit polyclonal
别名:
Anti-Anti-ANFH, Anti-Anti-AOM, Anti-Anti-COL11A3, Anti-Anti-SEDC, Anti-Anti-STL1
产品名称
Anti-Collagen Type II Antibody, Chemicon®, from rabbit
biological source
rabbit
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... COL2A1(1280)
Application
Anti-Collagen Type II Antibody detects level of Collagen Type II & has been published & validated for use in ELISA, WB, IC, IH.
ELISA 1:1000.
Indirect immunofluorescence on cryostat sections or cultured cells 1:10-1:20
Western Blot 1:100
Optimal working dilutions must be determined by the end user.
Indirect immunofluorescence on cryostat sections or cultured cells 1:10-1:20
Western Blot 1:100
Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
ECM Proteins
ECM Proteins
Biochem/physiol Actions
The antibody reacts with both native and denatured human collagen type II.
Cross Reactivity Percent
Human collagen type II 90%
Human collagen type I, III, IV, V <1%
Human plasma proteins do not interfere with binding to collagen.
Cross Reactivity Percent
Human collagen type II 90%
Human collagen type I, III, IV, V <1%
Human plasma proteins do not interfere with binding to collagen.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Immunogen
Collagen type II extracted and purified from human cartilage.
Physical form
Rabbit antiserum passed over agarose sorbents with immobilized human blood plasma proteins and human collagen types I, III, IV and V. Antibody was affinity purified on immobilized human collagen type II.
Product is lyophilized.
Product is lyophilized.
Preparation Note
Maintain at 2-8°C for up to 12 months from date of reciept. Remove any nondispersed aggregates by microcentrifugation prior to use.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Danger
hcodes
Hazard Classifications
Repr. 1B
存储类别
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Gabriella Nilsson Hall et al.
Biomaterials, 273, 120820-120820 (2021-04-20)
Tissue engineered constructs have the potential to respond to the unmet medical need of treating deep osteochondral defects. However, current tissue engineering strategies struggle in the attempt to create patterned constructs with biologically distinct functionality. In this work, a developmentally-inspired
Gabriella Nilsson Hall et al.
Advanced science (Weinheim, Baden-Wurttemberg, Germany), 7(2), 1902295-1902295 (2020-01-30)
Clinical translation of cell-based products is hampered by their limited predictive in vivo performance. To overcome this hurdle, engineering strategies advocate to fabricate tissue products through processes that mimic development and regeneration, a strategy applicable for the healing of large
Hannah Agten et al.
Frontiers in bioengineering and biotechnology, 12, 1386692-1386692 (2024-04-26)
Osteochondral defects are deep joint surface lesions that affect the articular cartilage and the underlying subchondral bone. In the current study, a tissue engineering approach encompassing individual cells encapsulated in a biocompatible hydrogel is explored in vitro and in vivo.
Fu-Hui Wang et al.
Scientific reports, 12(1), 21663-21663 (2022-12-16)
The human type II collagen (Col II), specifically expressed in chondrocytes, is a crucial component of the adult hyaline cartilage. We examine the potential of artificial induction of Col II in human peripheral blood mononuclear cells (PBMNCs) as a novel
Mu-En Lin et al.
Cardiovascular research, 112(2), 606-616 (2016-09-28)
Vascular smooth muscle cells (SMCs) are major precursors contributing to osteochondrogenesis and calcification in atherosclerosis. Runt-related transcription factor-2 (Runx2) has been found essential for SMC differentiation to an osteochondrogenic phenotype and subsequent calcification in vitro. A recent study using a
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