Anti-Kv11.2 Antibody

FUNCTION: SwissProt: Q9H252 # Pore-forming (alpha) subunit of voltage-gated potassium channel. Elicits a slowly activating, rectifying current (By similarity). Channel properties may be modulated by cAMP and subunit assembly.
SIZE: 994 amino acids; 109925 Da
SUBUNIT: The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming alpha subunits that can associate with modulating beta subunits. Heteromultimers with KCNH2/ERG1 and KCNH7/ERG3 (By similarity).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Expressed in prolactin-secreting adenomas.
DOMAIN:SwissProt: Q9H252 The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
SIMILARITY: Belongs to the potassium channel family. H (Eag) subfamily. & Contains 1 cyclic nucleotide-binding domain. & Contains 1 PAC (PAS-associated C-terminal) domain. & Contains 1 PAS (PER-ARNT-SIM) domain.

Purified peptide from amino acids 185-198 of human K_v11.

Western blot: 1:200 using ECL on rat brain lysate and rat cortex lysate.

Immunohistochemistry on rat brain sections.

Dilutions should be made using a carrier protein such as BSA (1-3%).

Optimal working dilutions must be determined by the end user.

Recognizes Kv11.2 (erg2, Voltage-gated K⁺ channel subfamily H member 6, KCNH6, ether-a-go-go channel 2).

Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, and 0.025% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).

Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

The immunogen sequence is identical in chicken and 13 of 14 amino acids identical in mouse.

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