Cellular tumor antigen p53 is a member of the p53 family and is found in the cytoplasm, nucleus, and endoplasmic reticulum. It functions as a tumor suppressor within a variety of tumors by either stimulating apoptosis or growth arrest in deference to cell type and physiological factors. p53 serves as a trans-activator, negatively regulating cell division during cell cycle regulation. It is also thought to be involved in the cross-over for Notch signaling. Defects in p53 expression have been implicated in several diseases including; choroid plexus papilloma, lung cancer, head/neck squamous cell carcinomas, esophageal squamous cell carcinoma, Li-Fraumeni syndrome, and hereditary adrenocortival carcinoma. Mutations of the p53 protein have some characteristic features: a) Most of them are missense point mutations giving rise to an altered protein function, and b) Many -but not all- mutant p53 proteins exhibit a common mutant structure, which can be recognized by monoclonal antibodies specific for p53 in the mutant conformation.

This antibody recognizes p53 when glutathionylated at Cys141.

Epitope: p53 glutathionylated at Cys141

KLH-conjugated linear peptide corresponding to human p53 glutathionylated at Cys141.

Anti-glutathione p53 (Cys141) Antibody is a rabbit polyclonal antibody for detection of glutathione p53 (Cys141) also known as Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53 & has been validated in WB, IP, IHC, ICC.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Western Blot Analysis: A representative lot was used by an independent laboratory in WB. (Dr. Kalkunte S. Srivenugopal, Department of Biomedical Sciences, Texas Tech University Health Sciences Center, 1406 S. Coulter, Amarillo, TX 79106.)

Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in IP. (Yusef, M, et al. (2010). Free Radic Biol Med. 49(5):908-917.)

Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in IC. (Yusef, M, et al. (2010). Free Radic Biol Med. 49(5):908-917.)

Evaluated by Immunohistochemistry in prostate adenocarcinoma tissue.

Immunohistochemistry Analysis: A 1:500 dilution of this antibody detected glutathione p53 in prostate adenocarcinoma tissue.

~53 kDa observed

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
Prostate adenocarcinoma tissue

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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