Anti-WWP2 Antibody

WW2 (atrophin-1-interacting protein 2) is a member of a large family of NEDD4-like E3 ligases that transfer ubiquitin from E2 ubiquitin-conjugating enzymes to targeted protein substrates. In general, ubiquitination affects protein turnover and activity. As a result, ubiquitin-related enzymes play essential roles in many cellular processes. The WW2 E3 ubiquitin-protein ligase contains a C2 domain at the N-terminus and a HECT (E6AP-type E3 ubiquitin-protein ligase) domain at the C-terminus which interact to inhibit its catalytic activity. Previous studies have suggested that WW2 may play a role in cellular transport, transcription, and T-cell activation. It has also been shown that WW2 regulates the PTEN tumor suppressor and plays an essential role in apoptosis and survival of cancer cells. WW2-deficient mice show abnormal development of craniofacial structures.

~100 kDa observed. Uncharacterized bands may be observed at ~31 kDa, ~57 kDa and ~60 kDa in some cell lysates.

GST-tagged recombinant protein corresponding to mouse WWP2.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

This Anti-WWP2 Antibody is validated for use in Western Blotting, IHC for the detection of WWP2.

Western Blot Analysis: A representative lot of this antibody detected WWP2 in CGR8 ES whole cell lysate and in transiently transfected HEK293 cells (Xu, H. M. et al. (2004). J Biol Chem. 279(22):23495-23503.).

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
mESC lysate

Evaluated by Western Blot in mESC lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected WWP2 in 10 µg of mESC lysate (For optimal performance, a range of 1 to 2 µg/mL of this antibody is recommended in Western Blot).

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.