Bcl-2-related protein A1 (BCL2A1), also known as protein BFL-1, protein GRS, and Bcl-2-like protein 5 (Bcl2-L-5), is a member of the Bcl-2 family. BCL2A1/BFL-1 can interact with Bax and suppress apoptosis by inhibiting the release of cytochrome c and caspase-3 activation. BCL2A1/BFL-1 is expressed in peripheral blood, spleen, bone marrow, lung, small intestine, and testis.

~20 kDa observed. Uncharacterized band(s) may appear in some lysates.

Recombinant protein corresponding to human BCL2A1/BFL-1.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

This Anti-BCL2A1 Antibody/BFL-1 is validated for use in Western Blotting and Immunohistochemistry and Immunoprecipitation for the detection of BCL2A1/BFL-1.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected BCL2A1/BFL-1 in 0.2 µg of Jurkat cell lysate.
Western Blotting Analysis: A representative lot detected BCL2A1/BFL-1 in human lymphoma cell lysate (Yecies, D., et al. (2010). Blood. 115(16):3304-13).
Western Blotting Analysis: A representative lot detected BCL2A1/BFL-1 in chronic lymphocytic leukemia cell lysate (Natoni, A., et al. (2011). Mol Cancer Ther. 10(9):1624-1634).
Immunohistochemistry Analysis: A representative lot detected BCL2A1/BFL-1 in lymph node tissue (Vogler, M., et al. (2009). Blood. 113(18): 4403-4413).
Immunoprecipitation Analysis: A representative lot immunoprecipitated BCL2A1/BFL-1 in chronic lymphocytic leukemia cell lysate (Vogler, M., et al. (2009). Blood. 113(18): 4403-4413).

Unpurified

Rabbit polyclonal serum with 0.05% sodium azide.

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Evaluated by Western Blotting in human BFL-1 recombinant protein.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected BCL2A1/BFL-1 in 0.2 µg of human BFL-1 recombinant protein.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.