Endostatin is a proteolytic cleavage product from the C-terminus of Collagen XVIII, which is itself a proteoglycan/collagen found in blood vessel walls and in basement membranes. Endostatin is a compact globular fold-containing protein with a Zn2+ binding site near its amino terminus. Originally identified in conditioned media of murine hemangioendothelioma (EOMA) cells, endostatin is generated from the parent collagen XVIII molecule involving intermediate glycosylated forms and the proteolytic activity of proteases such as cathepsin L, metalloproteases, and elastase. Endostatin has a wide-spread tissue expression and has been shown to co-localize with fibulins, nidogen-2 in basement membranes, and elastic fibers of veins. Endostatin has also been shown to cause a G1 arrest in endothelial cells and to induce apoptosis.

~28 kDa observed.
A ~28 kDa endostatin fragment has been observed in western blot as a result of MMP-7 cleavage of collagen XVIII (Chang, J., et al. (2003). Invest Ophthalmol Vis Sci. 44:832).

Epitope: Nonhelical Region 11 Domain

Linear peptide corresponding to the nonhelical region 11 of mouse Endostatin.

Anti-Endostatin Antibody detects level of Endostatin & has been published & validated for use in WB, IH(P).

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Endostatin in mouse lung tissue.

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

This antibody recognizes Endostatin at the nonhelical region 11 domain.

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
A431 cell lysate

Evaluated by Western Blot in A431 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Endostatin in 10 µg of A431 cell lysate.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: AB1880

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