ABD16
Anti-IKAROS Antibody
from rabbit, purified by affinity chromatography
别名:
DNA-binding protein Ikaros, Ikaros family zinc finger protein 1, Lymphoid transcription factor LyF-1
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ICC, WB
Citations:
-
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
rat, human, mouse
technique(s)
immunocytochemistry: suitable, western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... IKZF1(10320)
mouse ... Ikzf1(22778)
rat ... Ikzf1(305501)
General description
IKAROS, also known as IKZF1, is a transcription factor that is important for the regulation of lymphopoiesis and also the development of the immune system. It has also been linked to adult and fetal erythroid differentiation in humans. IKAROS has been shown to induce local chromatin accessibility during CD4+ T cell differentiation and therefore, activating Th2 expression.
~65 kDa observed. (Martin-Ibanez, R., et al. (2010). The Journal of Comparative Neurology. 518:329–351.) An uncharacterized band appears at ~57 kDa in some lysates.
Immunogen
KLH-conjugated linear peptide corresponding to human IKAROS.
Application
Anti-IKAROS Antibody detects level of IKAROS & has been published & validated for use in WB & IC.
Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected IKAROS in ReNcell human neural progenitor cells.
Research Category
Stem Cell Research
Stem Cell Research
Research Sub Category
Transcription Factors
Transcription Factors
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
HeLa cell lysate
HeLa cell lysate
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected IKAROS on 10 µg of HeLa cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected IKAROS on 10 µg of HeLa cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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