Anti-Chromobox protein homolog 5 Antibody

Chromobox protein homolog 5, also known as Antigen p25, Heterochromatin protein 1 homolog alpha (HP1 alpha), and encoded by the gene name CBX5 or HP1A is a component of heterochromatin that recognizes and binds histone H3 tails methylated at ′Lys-9′ (H3K9me), leading to epigenetic repression. In contrast, Chromobox protein homolog 5 is excluded from chromatin when ′Tyr-41′ of histone H3 (H3Y41ph) is phosphorylated. CBX5 is implied to interact with lamin-B receptor (LBR), which can contribute to the association of the heterochromatin with the inner nuclear membrane. Recent studies have shown that CBX5 is involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1α, HP1β and HP1γ). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated.

~23 kDa observed

BCP-conjugated linear peptide corresponding to human Chromobox protein homolog 5.

Immunocytochemistry Analysis: 2.0 µg/mL from a representative lot detected Chromobox protein homolog 5 in HeLa and A431 cells.
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Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

This Anti-Chromobox protein homolog 5 Antibody is validated for use in Western Blotting and Immunocytochemistry for the detection of Chromobox protein homolog 5.

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in HeLa nuclear extract.

Western Blotting Analysis: 0.1 µg/mL of this antibody detected Chromobox protein homolog 5 in 10 µg of HeLa nuclear extract.

Concentration: Please refer to lot specific datasheet.

ALEXA FLUOR is a trademark of Life Technologies

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