Anti-monomethyl-Histone H3 (Lys9) Antibody, Trial Size

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

~ 17 kDa

Epitope: Lys9

KLH-conjugated branched peptide corresponding to human Histone H3 methylated at Lys9.

Anti-monomethyl-Histone H3 (Lys9) Antibody, Trial Size is a rabbit polyclonal for detection of Histone H3 monomethylated on lysine 9. Also known as H3K9me1, this antibody has dot blot proven specificity, and has been validated in WB, ICC, PIA. Small pack size is ideal for screening.

Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected Histone H3 in HeLa and A431 cells.

Peptide Inhibition Analysis: 1:10,000 dilution from a representative lot was used with HeLa acid extract for peptide block analysis.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Broad species cross-reactivity is expected based on sequence homology.

This antibody recognizes Histone H3 methylated at Lys9.

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
HeLa acid extract lysate

Evaluated by Western Blot in HeLa acid extract lysate.

Western Blot Analysis: 0.1 µg/ml of this antibody detected Histone H3 on 10 µg of HeLa acid extract lysate.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: 07-395

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