Anti-PRMT7 Antibody

PRMT7, also known as Protein arginine N-methyltransferase 7, Histone-arginine N-methyltransferase PRMT7 or Myelin basic protein-arginine N-methyltransferase PRMT7, and encoded by the gene PRMT7/KIAA1933, is an arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. PRMT7 specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins SmD1 (SNRPD1) and SmD3 (SNRPD3). Such methylation is required for the assembly and biogenesis of snRNP core particles. PRMT7 also mediates the symmetric dimethylation of histone H4 ′Arg-3′ to form H4R3me2s, and it plays a role in gene imprinting by being recruited by CTCFL at the H19 imprinted control region (ICR) and methylating histone H4 to form H4R3me2s and thus leading to the recruitment of DNA methyltransferases to those sites. Finally, PRMT7 may also play a role in embryonic stem cell (ESC) pluripotency and is able to methylate histone H2A and myelin basic protein (MBP).

~75 kDa observed. Uniprot describes four isoforms at 79 kDa (Isoform 1), 62 kDa (Isoform 2), 73 kDa (Isoform 3), and 64 kDa (Isoform 4).

Epitope: unknown

GST-tagged recombinant protein corresponding to human PRMT7

Anti-PRMT7 Antibody detects level of PRMT7 & has been published & validated for use in PRMT7.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

This antibody is expected to cross react with all isoforms based upon immunogen design.

Affinity purified

Purified rabbit Polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in U2OS nuclear extract.

Western Blotting Analysis: A 1:500 dilution of this antibody detected PRMT7 in 10 µg of U2OS nuclear extract.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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