Chromobox protein homolog 3, also known as Chromobox protein homolog 3 (HECH), Heterochromatin protein 1 homolog gamma (HP1 gamma), or Modifier 2 protein, and encoded by the gene name CBX3, is implied to be involved in transcriptional silencing in heterochromatin-like complexes. Chromobox protein homolog 3 recognizes and binds histone H3 tails methylated at ′Lys-9′, leading to epigenetic repression. Chromobox protein homolog 3 may contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Chromobox protein homolog 3 may also play a role in the formation of functional kinetochore through interaction with MIS12 complex proteins. Chromobox protein homolog 3 binds directly to CHAF1A and it has been shown to interact with LBR, INCENP, TRIM28/TIF1B, SUV420H1, SUV420H2, SP100, MIS12, DSN1, CBX5 via the chromoshadow domain, POGZ, CHAMP1, and ASXL1. Chromobox protein homolog 3 is phosphorylated by PIM1. Recent studies have implied that Chromobox protein homolog 3 is phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.

Epitope: Chromo Domain 1

KLH-conjugated linear peptide corresponding to Chromo Domain 1 of human Chromobox protein homolog 3.

Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated Chromobox protein homolog 3 in 500 µg of HeLa nuclear extract.
Immunocytochemistry Analysis: 1.0 µg/mL from a representative lot detected Chromobox protein homolog 3 in HeLa cells.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

This Anti-Chromobox protein homolog 3 antibody is validated for use in WB, IC;IP for the detection of Chromobox protein homolog 3.

Evaluated by Western Blotting in HeLa nuclear extract.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Chromobox protein homolog 3 in 10 µg of HeLa nuclear extract.

~22 kDa observed

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to lot specific datasheet.

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