一般描述
95 kDa and 105 kDa
Sp1 was one of the first transcription factors to be characterized in mammalian cells. The Sp1 protein plays a role in the regulation of gene expression. This protein has three Cys2His2 zinc-finger motifs that have been shown to bind to the consensus sequence 5′-(G/T)GGGCGG(G/A)(G/A)(C/T)-3′ also known as a GC box. Sp1 is a ubiquitous transcription factor required for the expression of a variety of genes, such as those involved in the cell cycle and development. Interaction of the Sp1 protein with the GC box activates transcription of specific mRNAs from genes that contain functional recognition sites.
免疫原
GST-tagged recombinant protein corresponding to full-length human Sp1.
应用
Anti-Sp1 Antibody is a Rabbit Polyclonal Antibody for detection of Sp1 also known as Sp1 transcription factor, specificity protein 1 & has been validated in WB, IP, ICC.
Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected Sp1 in HeLa and A431 cells.
Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated Sp1 from 500 µg of HeLa nuclear extract.
Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated Sp1 from 500 µg of HeLa nuclear extract.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
生化/生理作用
Homology to chimpanzee, canine, and rhesus monkey is 99%.
This antibody recognizes Sp1.
外形
Protein A purified
Format: Purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
制备说明
Stable for 1 year at 2-8°C from date of receipt.
分析说明
Control
HeLa nuclear extract
HeLa nuclear extract
Evaluated by Western Blot in HeLa nuclear extract.
Western Blot Analysis: 0.1 µg/mL of this antibody detected Sp1 on 10 µg of HeLa nuclear extract.
Western Blot Analysis: 0.1 µg/mL of this antibody detected Sp1 on 10 µg of HeLa nuclear extract.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: MABE550
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
未找到合适的产品？
试试我们的产品选型工具.
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
K A Gajewska et al.
Nature communications, 12(1), 5904-5904 (2021-10-10)
The importin superfamily member Importin-13 is a bidirectional nuclear transporter. To delineate its functional roles, we performed transcriptomic analysis on wild-type and Importin-13-knockout mouse embryonic stem cells, revealing enrichment of differentially expressed genes involved in stress responses and apoptosis regulation.
Adrien Morel et al.
Oncotarget, 8(28), 46163-46176 (2017-05-19)
High-risk human papillomaviruses are the etiological agents of cervical cancer and HPV16 is the most oncogenic genotype. Immortalization and transformation of infected cells requires the overexpression of the two viral oncoproteins E6 and E7 following HPV DNA integration into the
Christoph Hoffmann et al.
PloS one, 8(12), e83426-e83426 (2014-01-07)
Uncoupling protein (UCP) 3 is a mitochondrial inner membrane protein implicated in lipid handling and metabolism of reactive oxygen species. Its transcription is mainly regulated by peroxisome proliferator-activated receptors (PPAR), a family of nuclear hormone receptors. Employing bandshift assays, RNA
Lina Adwan et al.
Neuropharmacology, 79, 596-602 (2014-01-28)
Environmental exposure to lead (Pb) early in life results in a latent upregulation of genes and products associated with Alzheimer's disease (AD), particularly the plaque forming protein amyloid beta (Aβ). Furthermore, animals exposed to Pb as infants develop cognitive decline
Sandra Kissing et al.
Autophagy, 13(4), 670-685 (2017-01-28)
The vacuolar-type H+-translocating ATPase (v-H+-ATPase) has been implicated in the amino acid-dependent activation of the mechanistic target of rapamycin complex 1 (MTORC1), an important regulator of macroautophagy. To reveal the mechanistic links between the v-H+-ATPase and MTORC1, we destablilized v-H+-ATPase
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持