NuMA1 or NuMA protein is an interesting and major component of the nuclear matrix of eukaryotic cells. NuMA1 is a highly abundant protein component that appears to serve a non-structural role but is needed for mitotic spindle poles and centrosome formation as well as a linker of microtubules. These functions are important for proper cytokinesis and the setup of the proper polarity axis during cell division. NuMA1 resides in the nuclear matrix during interphase. NuMA1 dissociates from condensing chromosomes during early prophase, and relocates to the spindle poles via a dynein/dynamin complex, and it remains there until the anaphase onset. Thus NuMA1 then can be found in the nucleus during cell rest and cytoplasmically and centrosome localized during cytokinesis.

~ 238 kDa observed

Three different peptides corresponding to multiple locations of NuMA1 isoform 1. Peptide, region between amino acids 1-240. Peptide 2, amino acids 1300-1500. Peptide 3, amino acids 1860-2115. Note that these peptides are also found in isoform 2.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot was reported to detect NuMA1 in HeLa cells.

Research Category
Epigenetics & Nuclear Function

Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Chromatin Biology

This Anti-NuMA1 Antibody is validated for use in Western Blotting and Immunocytochemistry for the detection of NuMA1.

Recognizes NuMA1

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS, 50% glycerol, and 0.05% sodium azide.

Store at -20°C

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: A 1 µg/mL dilution of this antibody detected NuMA1 in a A431 cell lysate.

Concentration: Please refer to lot specific datasheet.

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