Anti-RAD18 Antibody

GST-tagged recombinant mouse RAD18 C-terminal fragment.

~60/70 kDa observed. Given the difference of about 10 kDa in the molecular mass of the two
proteins, we concluded that the upper band was derived from monoubiquitination of the lower band (Miyase, S., et al. (2005). J. Biol. Chem. 280:515-524). Uncharacterized band(s) may appear in some lysates.

Epitope: C-terminal region.

This polyclonal antiserum specifically detected RAD18 expression in wild-type, but not Rad18-/-, mouse embryonic fibroblasts (MEFs) by Western blotting and fluorescent immunocytochemistry (Tateishi, S., et al. (2003). Mol. Cell. Biol. 23(2):474-481).

Rabbit polyclonal serum with 0.02% sodium azide.

Stable for 1 year at -20°C from date of receipt.Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Evaluated by Western Blotting in MEF-1 cell lysate.

Western Blotting Analysis: A 1:500 dilutiion of this antibody detected RAD18 in 10 µg of MEF-1 cell lysate.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.