Anti-phospho Histone H3.1/H3.2 Antibody (Ser87)

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone H3.1, H3.2 and H3.3 are all closely related Histone H3 variants, that despite differing by only a few amino acids have been shown to have different functional roles.

~17 kDa observed

Epitope: Phosphorylated at Ser87

KLH-conjugated linear peptide corresponding to human Histone H3.1/H3.2 phosphorylated at Ser87.

Detect Histone H3 using this rabbit polyclonal antibody, Anti-phospho Histone H3.1/H3.2 Antibody (Ser87) validated for use in western blotting & Dot Blot.

Dot Blot (Specificity) Analysis: 1µg/mL of this antibody detected phospho Histone H3.1/H3.2 (Ser87) in a panel of unmodified and modified Histones.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

This antibody recognizes Histone H3.1/H3.2 phosphorylated at Ser87. This antibody demonstrates specificity for phospho Histone H3.1/H3.2 (Ser87), but does not cross react with phospho Histone H3.3, or any other modified and non-modified histones.

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
HeLa acid extract

Evaluated by Western Blotting in Colcemid treated HeLa acid extract.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected phospho Histone H3.1/H3.2 (Ser87) in 10 µg of Colcemid treated HeLa acid extract

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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