antibody form
purified antibody
clone
polyclonal
form
liquid
purified by
affinity chromatography
species reactivity
rabbit, mouse, human
concentration
(Please refer to lot specific datasheet.)
technique(s)
ChIP: suitable, dot blot: suitable, western blot: suitable
isotype
IgG
UniProt accession no.
General description
KLH-conjugated linear peptide corresponding to 10 amino acids from the N-terminal region of human Histone H3.1 serotonylated on glutamine 5.
~16 kDa observed; 15.51 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Application
Epitope: N-terminus
Biochem/physiol Actions
This rabbit polyclonal antibody specifically detects Histone H3.1 serotonylated at glutamine 5 position.
Physical form
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Dot Blot on H3Q5-Serotonyl peptide.
Dot Blot Analysis: A 1:500 dilution of this antibody detected Histone H3Q5serH3Q5-Serotonyl peptide, but not the unmodified H3(1-10)-control peptide.
Dot Blot Analysis: A 1:500 dilution of this antibody detected Histone H3Q5serH3Q5-Serotonyl peptide, but not the unmodified H3(1-10)-control peptide.
Other Notes
Human, Mouse.
Disclaimer
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