Anti-Histone H3Q5ser

Histone H3.1t (UniProt: Q16695; also known as H3/t, H3t, H3/g) is encoded by the HIST3H3 (also known as H3FT) gene (Gene ID: 8290) in human. Histone H3 has two main variants, H3.1 and H3.3, which show different genomic localization patterns in animals. The H3.1 and H3.3 complexes also possess distinct histone chaperones, CAF-1 and HIRA, which play important role in mediating DNA-synthesis-dependent and -independent nucleosome assembly. Histone H 3.1 is a core component of nucleosome that is present only in mammals. It is expressed during S phase, then its expression decreases significantly as cell division slows down during the process of differentiation. Histone H 3.1 expression is shown to be replication dependent. It serves as the canonical histone, which is incorporated during DNA replication. Histones are dynamic proteins that undergo multiple types of post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination. Histone 3.1 is also shown to undergo serotonylation of glutamine at position 5 (Q5ser) in organisms that produce serotonin. It has been reported that Histone H3 can also undergo dopaminylation ((H3Q5dop), which can potentiate the binding of adjacent histone post-translational modification interacting proteins and play a vital role in dopaminergic neuronal transcription. This rabbit polyclonal antibody specifically binds to Histone H3 dopaminylated on glutamine 5. (Ref.: Lepack, AE., et al (2020). Science. 368(6487); 197-201).

KLH-conjugated linear peptide corresponding to 10 amino acids from the N-terminal of human Histone H3.1 serotonylated at Glutamine 5.

Target molecular weight ~ 16 kDa observed. 15.508 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Epitope: N-terminus

Anti-Histone H3Q5ser is a rabbit polyclonal antibody that detects Histone H3 serotonylated at Glutamine 5. It targets an epitope within 10 amino acids from the N-terminal.

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Recommended storage: +2°C to +8°C.

Evaluated by dot blot with unmodified and Histone H3Q5-serotonyl peptides.

Dot Blot Analysis: A 1:2,000 dilution from a representative lot detected Histone H3Q5-serotonyl peptide but not the unmodified Histone H3 control peptide (aa 1-10).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Human, Mouse.

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