一般描述
Adenosylhomocysteinase (UniProt: P23526; also known as EC: 3.3.1.1, AdoHcyase, S-adenosyl-L-homocysteine hydrolase) is encoded by the AHCY (also known as SAHH) gene (Gene ID: 191) in human. Adenosylhomocysteinase is a homotetrametic enzyme that catalyzes the reversible hydrolysis of S-adenosylhomocysteine to adenosine and L-homocysteine. Hence, it regulates the intracellular S-adenosylhomocysteine concentration, which is thought to be important for transmethylation reactions. Two isoforms of adenosylhomocysteinase have been reported that are produced by alternative splicing. It contains three nucleotide binding regions (aa 157-159; 222-227; and 299-301). Defects in AHCY gene cause hypermethioninemia that is characterized with failure to thrive, mental and motor retardation, facial dysmorphism with abnormal hair and teeth, and myocardiopathy.
特异性
This rabbit polyclonal antibody detects Adenosylhomocysteinase in human. It targets and epitope within 12 amino acids from the C-terminus region.
免疫原
Epitope: C-terminus
KLH-conjugated linear peptide corresponding to 12 amino acids from the C-terminus region of human Adenosylhomocysteinase.
应用
Anti-Adenosylhomocysteinase (AHCY) Antibody, Cat. No. ABE1803, is a highly specific rabbit polyclonal antibody that targets Adenosylhomocysteinase (AHCY) and has been tested for use in Immunohistochemistry (Paraffin), and Western Blotting.
Research Category
Epigenetics & Nuclear Function
Western Blotting Analysis: 2 µg/mL from a representative lot detected Adenosylhomocysteinase (AHCY) in 200 µg of human placental tissue lysate.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Adenosylhomocysteinase (AHCY) in human kidney and human skin tissues.
质量
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected Adenosylhomocysteinase (AHCY) in 200 µg of HeLa cell lysate.
目标描述
~47 kDa observed; 47.72 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
外形
Affinity Purified
Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, pH 7.4, with 0.05% sodium azide.
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
其他说明
Concentration: Please refer to lot specific datasheet.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
