Cyclin-dependent kinase 13 (UniProt: Q14004: also known as Cdk13, EC:2.7.11.22, EC:2.7.11.23, CDC2-related protein kinase 5, Cell division cycle 2-like protein kinase 5, Cell division protein kinase 13, hCDK13, Cholinesterase-related cell division controller) is encoded by the CDK13 (also known as CDC2L, CDC2L5, CHED, KIAA1791) gene (Gene ID:8621) in human. CDK13 is a member of the cyclin-dependent serine/threonine protein kinase family. Members of this family are known to play a vital role as master switches in cell cycle control. Human CDK 13 shares 43% sequence identity with CDK12. CDK13 is expressed in fetal brain, liver, muscle, in adult brain and in neuroblastoma and glioblastoma tumors. It contains expanded regions of serine-arginine (SR) motifs in its N-terminal regions, which links it to the SR protein family involved in RNA processing and pre-mRNA splicing. CDK13 contains a C-terminal extension helix that is composed of a polybasic cluster and a DCHEL motif that interacts with the bound ATP. CDK13 displays C-terminal repeat domain (CTD) kinase activity and is required for RNA splicing. It hyperphosphorylates the C-terminal heptapeptide repeat domain of the largest RNA polymerase II subunit RPB1 and acts a key regulator of transcription elongation. Phosphorylation of the three serine residues (Ser 2, 5, and 7) within the CTD is linked to the phases of RNAPII-mediated transcription. Phosphorylation of Ser7 (pSer7) starts the transcription cycle that primes the CTD for further modifications. (Ref.:Greifenberg, AK et al. (2016). Cell Reports 14(2): 320-331).

~170 kDa observed; 164.92 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

KLH-conjugated linear peptide corresponding to 19 amino acids from the N-terminal half of human Cyclin-dependent kinase 13 (CDk13).

Anti-CDK13, Cat. No. ABE1860, is a highly specific rabbit polyclonal antibody that targets Cyclin-dependent kinase 13 and has been tested in Western Blotting.

Research Category
Epigenetics & Nuclear Function

Western Blotting Analysis: A representative lot detected CDK13 in HeLa cell lysate (Bartkowiak, B., et. al. (2010). Genes Dev. 24(20):2303-16).

Western Blotting Analysis: 1:1,000 dilution from a representative lot detected CDK13 in HEK293 whole cell extract. (Courtesy of Dr. A. Greenleaf, Duke University Medical Center).

Western Blotting Analysis: 1:1,000 dilution from a representative lot detected CDK13 in HeLa nuclear extract. (Courtesy of Dr. A. Greenleaf, Duke University Medical Center).

Western Blotting Analysis: 1:1,000 dilution from a representative lot detected CDK13 in OVCA5 and HEK293 (Courtesy of Dr. A. Greenleaf, Duke University Medical Center).

This polyclonal antibody specifically detects CDK13 in HEK293 cells.

Affinity Purified

Format: Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in HEK293 whole cell extract.

Western Blotting Analysis: 1:500 dilution of this antibody detected CDK13 in 20 µL of HEK293 whole cell extract.

Concentration: Please refer to lot specific datasheet.

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