Anti-Set1A Antibody

Set1A is a histone methyltransferase (HMT) that is part of the SET1 HMT complex. This protein specifically methylates lysine 4 of histone H3 if the adjacent lysine at position 9 is also unmethylated. Studies demonstrate that Set1A and Set1B localize to largely non-overlapping regions of the chromosome. This suggests Set1A and Set1B bind to unique sets of target genes and that each protien makes non-redundant contributions to the epigenetic control of chromatin structure and gene expression.

~186 kDa observed. A cross-reacting band appears at ~45 kDa

GST-tagged recombinant protein corresponding to human Set1A.

Chromatin Immunoprecipitation (ChIP):
A representative lot of this antibody was reported to work in ChIP. See Lee, J.H., et al.(2008). Mol Cell Biol. 28(2):609–618.

Immunocytochemistry:
A representative lot of this antibody was reported to work in immunocytochemistry. See . Lee, J.H., et al. (2007). J Biol Chem. 282(18):13419-13428.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histone Modifying Proteins

Use Anti-Set1A Antibody (Rabbit Polyclonal Antibody) validated in WB to detect Set1A also known as SET domain containing 1A, Set1/Ash2 histone methyltransferase complex subunit SET1.

Rabbit polyclonal serum containing 0.05% sodium azide.

Unpurified

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Control
PC3 cell lysate

Evaluated by Western Blot in PC3 cell lysate.

Western Blot Analysis: 1:1,000 dilution of this antibody detected Set1A on 10 µg of PC3 cell lysate.

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