The protein called Friend of PRMT1 (FOP), or more correctly Chromatin target of PRMT1 protein, or Small arginine and glycine rich protein (SRAG) is an important partner in estrogen receptor gene activation events and plays a role in 5FMC mediated desumoylation of ZNF148 and the subsequent gene activations of its target genes. Friend of PRMT1 also interacts with other methyltransferases such as PRMT5. The protein is also a component of the dynamic TREX mRNA export complex where it assists in mRNA export. The protein is expressed in the nucleus and contained in the nucleolus of various cell types including erythroid progenitor cells.

Epitope: C-terminus

GST-tagged recombinant protein corresponding to the C-terminus of human Friend of PRMT1.

Anti-Friend of PRMT1 Antibody is an antibody against Friend of PRMT1 for use in western blotting & ChIP.

Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory detected Friend of PRMT1 in MCF-7 cell lysate (Van Dijk, T. B., et al. (2010). Mol Cell Biol. 30(1):260-272.).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Friend of PRMT1 in 10 µg of A431 cell lysate.

~32 kDa observed

Unpurified

Rabbit polyclonal serum with 0.05% sodium azide.

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

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