Anti-Histone H3Kme3Q5Ser

Histone H3.1 UniProt: Q16695; also known as H3/t, H3t, H3/g) is encoded by H3-4 (also known as H3FT, HIST3H3) gene (Gene ID: 8290) in human. Histone H3 has two main variants, H3.1 and H3.3, which show different genomic localization patterns in animals. Histone H 3.1 is a core component of nucleosome that is present only in mammals. It is expressed during S phase, then its expression decreases significantly as cell division slows down during the process of differentiation. Histone H 3.1 expression is shown to be replication dependent. It serves as the canonical histone, which is incorporated during DNA replication. Histone 3.1 can also undergo serotonylation of glutamine at position 5 (Q5ser) in organisms that produce serotonin. This has been demonstrated that tissue transglutaminase 2 can serotonylate histone H3K4me3 marked nucleosomes, which results in the formation of H3K4me3Q5ser. The presence of Q5ser does not affect the ability of MLL1, an H3K4 methyltransferase, to modify nucleosomes in vitro. In the brain the H3K4me3Q5ser signal is not segregated only to regions in which serotonin is produced, but is shown to be broadly distributed. H3K4me3Q5ser expression has also been reported in human pluripotent stem cell-derived serotonergic neurons and with differentiation the levels of H3K4me3Q5ser increase significantly. This polyclonal Anti-Histone H3Kme3Q5Ser is suitable for Western Blotting, Dot blotting, ChIP seq applications. (Ref.: Farrelly, L.A., et al. (2019). Nature. 567(7749):535-539).

Target molecular weight ~16 kDa observed; 15.51 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

KLH-conjugated linear peptide corresponding to 10 amino acids from the N-terminal region of human Histone H3 trimethylated at lysine 4 and serotonylated at glutamine 5.

Epitope: N-terminus

Tested Applications

Western Blotting Analysis: A 1:500 dilution from a representative lot detected Histone H3 trimethylated at Lys4 and serotonylated at Gln5 in whole cell lysate from differentiated RN46A-B14 cells but not in lysate from undifferentiated RN46A-B14 cells.

ChIP-Seq Analysis: A representative lot of this antibody was used to assess genome-wide impacts of serotonergic differentiation in differentiated RN46A-B14 cells (Farrelly, LA., et al. (2109). Nature. 567(7749); 535-539).

Western Blotting Analysis; A representative lot of this antibody was used to detect Histone H3 trimethylated at Lys4 and serotonylated at Gln5 by Western Blotting application. (Farrelly, LA., et al. (2109). Nature. 567(7749); 535-539).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Anti-Histone H3Kme3Q5Ser, Cat. No. ABE2580, is a rabbit polyclonal antibody that detects Histone H3Kme3Q5Ser and is tested for use in Dot Blot and ChIP-seq, and Western Blotting.

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Recommended storage: +2°C to +8°C.

Evaluated by Dot Blot with unmodified and modified Histone H3Kme3Q5Serotonyl peptides.

Dot Blot Analysis: A 1:500 dilution from a representative lot detected Histone H3 trimethylated at Lys4 and serotonylated at Gln5 but not Histone H3(1-10) control synthetic peptide.

Human, Mouse.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.