As a component of the nuclear RNA cap-binding complex (CBC), Ars2 is known to play a critical role in miRNA biogenesis by stabilizing and linking capped primary miRNA transcripts with the primary miRNA processing complex containing DROSHA and DGGR8. Unlike other CBC components, Ars2 expression also plays an important role in cell proliferation. In particular, depletion of Ars2 is shown to reduce the levels of several miRNAs, such as miR-21, let-7, and miR-155, which are implicated in cellular transformation.

Linear peptide corresponding to human Ars2.

Immunoprecipitation Analysis: A representative lot from an independent laboratory detected Ars2 in IP. (Alex Achille, Department of Molecular Oncology, H. Lee Moffitt Cancer Center & Research Institute, Tampa, Florida, USA).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
RNA Metabolism & Binding Proteins

Use Anti-Ars2 Antibody (Rabbit Polyclonal Antibody) validated in WB, IP to detect Ars2 also known as Serrate RNA effector molecule homolog, Arsenite-resistance protein 2.

Evaluated by Western Blot in HEK293 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Ars2 in 10 µg of HEK293 cell lysate.

~120 kDa observed. Uncharacterized bands at ~78 kDa and 48 kDa may be observed in some lysates.

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
HEK293 cell lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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