Anti-CREB-H/CREB3L3

Cyclic AMP-responsive element-binding protein 3-like protein 3 (UniProt: Q68CJ9; also known as cAMP-responsive element-binding protein 3-like protein 3, Transcription factor CREB-H) is encoded by the CREB3L3 (also known as CREBH, HYST1481) gene (Gene ID: 84699) in human. CREB-H is a single-pass type II membrane protein that is exclusively expressed in the liver. It contains a cytoplasmic domain (aa 1-322), a transmembrane domain (aa 323-343), and a lumenal domain (aa 344-461). It is a RIP-regulated liver-specific transcription factor that is cleaved upon endoplasmic reticulum (ER) stress and is required to activate expression of acute phase response genes. In response to ER stress, CREB-H is cleaved by site-1 and site-2 proteases to liberate an N-terminal fragment that transits to the nucleus to activate transcription of the genes encoding serum amyloid P-component and C-reactive protein. Proinflammatory cytokines are also shown to increase the expression of ER membrane-anchored CREB-H. It is activated in response to cAMP stimulation and is reported to bind to the cAMP response element (CRE) and box-B element. It plays a crucial role in the regulation of triglyceride metabolism and is required for the maintenance of normal plasma triglyceride levels. CREB-H is shown to interact with PPAR to regulate FGF21, a hepatic hormone that regulates whole-body energy homeostasis. CREB-H and PPAR are reported to act as interactive trans-activators that regulate each other for their expression. They form a functional complex upon fasting or the atherogenic high-fat diet. CREB-H-deficient mice display hypertriglyceridemia secondary to inefficient triglyceride clearance catalyzed by lipoprotein lipase. (Ref.: Kim, H., et al. (2014). Endocrinology. 155(3);769-782; Lee, JH., et al. (2011). Nat. Med. 17(7); 812-815; Zhang, K., et al. (2006). Cell. 124(3); 587-599).

GST/His-tagged recombinant fragment corresponding to 92 amino acids from the internal region of human Cyclic AMP-responsive element-binding protein 3-like protein 3 (CREB-H).

Quality Control Testing

Evaluated by Western Blotting in lysate from wild-type Mouse liver tissue.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected CREB-H in lysate from wild-type Mouse liver tissue, but not in lysate from Mouse liver with CREB-H knockout.

Tested Applications

Chromatin Immunoprecipitation Analysis (ChIP): A representative lot detected CREB-H/CREB3L3 in Chromatin Immunoprecipitation applications. (Zhang, K., et al. (2006). Cell. 124(3); 587-599).

Western Blotting Analysis (WB): A representative lot detected CREB-H/CREB3L3 in Western Blotting applications. (Kim, H., et al. (2014). Endocrinology. 155(3); 769-782).

Immunohistochemistry Applications: A representative lot detected CREB-H/CREB3L3 in Immunohistochemistry applications. (Kim, H., et al. (2014). Endocrinology. 155(3); 769-782).

Immunofluorescence Analysis (IF): A representative lot detected CREB-H/CREB3L3 in Immunofluorescence applications ( (Zhang, K., et al. (2006). Cell. 124(3); 587-599).

Immunoprecipitation Analysis: A representative lot immunoprecipitated CREB-H/CREB3L3 in Immunoprecipitation applications. (Zhang, K., et al. (2006). Cell. 124(3); 587-599).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

This rabbit polyclonal antibody detects CREB-H (CREB3L3). It targets an epitope within the internal region.

Purified rabbit polyclonal antibody in PBS with 50% glycerol and without azide.

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.