Poly(A)-binding proteins, cytoplasmic 1 (PABPC1), and cytoplasmic 3 (PABPC3) are RNA-binding proteins with important roles in post-translational control and metabolism of mRNA. Although both proteins bind to the poly(A) tail of mRNA, PABPC3 binds with a lower affinity than PABPC1. Studies have associated these proteins with tumorigenesis. Decreased expression of PABPC1 in esophageal cancer seems to associate with tumor development and poor prognosis after surgery. PABPC1 can shuttle through the nuclear membrane and is thought to associate with newly synthesized poly(A) transcripts. PABPC1 has also been linked to microRNA mediated silencing when in contact with GW182. PABPC3 is thought to be specific to human testis and controls RNA stability.

~71 kDa observed

KLH-conjugated linear peptide corresponding to human PABPC1/C3. The immunogen sequence used is 100% identical to both PABPC1 and PABPC3.

Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected PABPC1/C3 in NIH/3T3 and HeLa cells.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
RNA Metabolism & Binding Proteins

Use Anti-PABPC1/C3 (Rabbit Polyclonal Antibody) validated in ICC, WB to detect PABPC1/C3 also known as Polyadenylate-binding protein 1, PABP-1.

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
HeLa cell lysate

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected PABPC1/C3 on 10 µg of HeLa cell lysate.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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