ABE446
Anti-Monomethyl Histone H1 (Lys26) Antibody
from rabbit, purified by affinity chromatography
别名:
Histone H1.3, Histone H1c, Histone H1s-2
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
polyclonal
Species reactivity:
human
Application:
dot blot
immunoprecipitation (IP)
western blot
immunoprecipitation (IP)
western blot
Technique(s):
dot blot: suitable
immunoprecipitation (IP): suitable
western blot: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Citations:
1
Uniprot accession no.:
产品名称
Anti-Monomethyl Histone H1 (Lys26) Antibody, from rabbit, purified by affinity chromatography
biological source
rabbit
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human
technique(s)
dot blot: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
target post-translational modification
monomethylation (Lys26)
Quality Level
Gene Information
human ... HIST1H1D(3007)
Analysis Note
Evaluated by Western Blotting in HeLa acid extract.
Western Blotting Analysis: 0.25 µg/mL of this antibody detected Monomethyl Histone H1 (Lys26) in 10 µg of HeLa acid extract.
Western Blotting Analysis: 0.25 µg/mL of this antibody detected Monomethyl Histone H1 (Lys26) in 10 µg of HeLa acid extract.
Application
Detect Histone H1 using this rabbit polyclonal antibody, Anti-Monomethyl Histone H1 (Lys26) Antibody validated for use in western blotting, Dot Blot & IP.
Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated Monomethyl Histone H1 (Lys26) in 500 µg of HeLa acid extract.
Dot Blot Specificity Analysis: This antibody detected Monomethyl Histone H1 (Lys26) in in an Absurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and in an Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665).
Dot Blot Specificity Analysis: This antibody detected Monomethyl Histone H1 (Lys26) in in an Absurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and in an Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665).
Biochem/physiol Actions
This antibody recognizes the N-terminus of Monomethyl Histone H1 (Lys26) methylated at Lys26.
General description
Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome. The linker histone, H1, interacts with linker DNA between nucleosomes, and functions in the compaction of chromatin into 30nm chromatin fibers and higher order structures.
~32 kDa observed
Immunogen
KLH-conjugated linear peptide corresponding to the N-terminus of human Monomethyl Histone H1 (Lys26).
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Weigang Cui et al.
Molecular medicine reports, 22(2), 739-750 (2020-05-30)
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