ABE476
Anti-GAL4 Antibody (activation domain)
from rabbit, purified by affinity chromatography
别名:
Regulatory protein GAL4
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
western blot
Species reactivity:
yeast
Citations:
1
Technique(s):
western blot: suitable
Uniprot accession no.:
产品名称
Anti-GAL4 Antibody (activation domain), from rabbit, purified by affinity chromatography
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
yeast
technique(s)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
Saccharomyces cerevisiae ... Gal4(855828)
Analysis Note
Control
Gal4 positive and Gal4 negative yeast
Gal4 positive and Gal4 negative yeast
Evaluated by Western Blot in Gal4 positive and Gal4 negative yeast cell lysate.
Western Blot Analysis: A 1:160,000 dilution of this antibody detected Gal4 in 5 mg of Gal4 positive (+ recombinant Gal4) yeast cell lysate. This antibody also detected small mounts of endogenous Gal4 in Gal4 negative (- recombinant Gal4) yeast cell lysate.
Western Blot Analysis: A 1:160,000 dilution of this antibody detected Gal4 in 5 mg of Gal4 positive (+ recombinant Gal4) yeast cell lysate. This antibody also detected small mounts of endogenous Gal4 in Gal4 negative (- recombinant Gal4) yeast cell lysate.
Application
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Cell Cycle, DNA Replication & Repair
Use Anti-GAL4 (activation domain) Antibody (Rabbit Polyclonal Antibody) validated in WB to detect GAL4 (activation domain) also known as Regulatory protein GAL4.
Biochem/physiol Actions
This antibody recognizes the C-terminus of GAL4 (activation domain).
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
GAL4 is a transcriptional activator which controls the expression of genes involved in the catabolism of galactose. In addition to a DNA-binding domain, Gal4 contains two acidic regions: activating region I (aa148-238) and activating region II (aa768-881), which can both activate transcription when independently joined to the DNA-binding domain. The activation domains of Gal4 have been shown to interact with Gal80, TBP, SUG1, and ADA2, among other general transcription factors.
~28 kDa, ~38 kDa, ~77 kDa observed
Immunogen
Epitope: C-terminus
GST-tagged recombinant protein corresponding to the C-terminus of yeast GAL4 (activation domain).
Other Notes
Replaces: 06-283
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Yukiko Imai et al.
eLife, 9 (2020-10-14)
Meiotic recombination starts with the formation of DNA double-strand breaks (DSBs) at specific genomic locations that correspond to PRDM9-binding sites. The molecular steps occurring from PRDM9 binding to DSB formation are unknown. Using proteomic approaches to find PRDM9 partners, we
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