Anti-phospho IRF3 (ser386) Antibody

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Evaluated by Western Blotting in MCF7 cell lysate.

Western Blotting Analysis: 0.1 µg/mL of this antibody detected detected IRF3 (ser386) in 10 µg of Calyculin A treated MCF7 cell lysate.

Detect IRF-3 using this rabbit polyclonal antibody, Anti-phospho IRF3 (ser386) Antibody validated for use in western blotting.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Interferon regulatory factors (IRFs) comprise a family of transcription factors that function with the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection. IRF-3 can inhibit cell growth and plays a critical role in controlling the expression of genes in the innate immune response. In unstimulated cells, IRF-3 is present in the cytoplasm. Viral infection results in phosphorylation of IRF-3 and leads to its translocation to the nucleus where it activates promoters containing IRF-3-binding sites. Phosphorylation of IRF-3 occurs at a cluster of C-terminal serine and threonine residues (between 385 and 405) leading to its association with the p300/CBP coactivator protein that promotes DNA binding and transcriptional activity.

~50 kDa observed

Epitope: Near C-terminus

KLH-conjugated linear peptide corresponding to human IRF3 (ser386) near the C-terminus.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Stable for 1 year at 2-8°C from date of receipt.