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ABE543

Sigma-Aldrich

Anti-PRDM16 Antibody

from rabbit, purified by affinity chromatography

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别名:
PR domain zinc finger protein 16, PR domain-containing protein 16, Transcription factor MEL1, MDS1/EVI1-like gene 1
eCl@ss:
32160702
NACRES:
NA.41

生物来源

rabbit

质量水平

100

抗体形式

affinity isolated antibody

抗体产品类型

primary antibodies

克隆

polyclonal

形式

(Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide)

分子量

(Target molecular weight ~140 kDa observed; 140.25 kDa calculated. Uncharacterized bands may be observed in some lysate(s).)

纯化方式

affinity chromatography

种属反应性

human

种属反应性（根据同源性预测）

horse (based on 100% sequence homology), canine (based on 100% sequence homology), bovine (based on 100% sequence homology), nonhuman primates (based on 100% sequence homology), guinea pig (based on 100% sequence homology)

技术

immunohistochemistry: suitable ((Paraffin))
immunoprecipitation (IP): suitable
western blot: suitable

NCBI登记号

NP_071397

UniProt登记号

Q9HAZ2

运输

wet ice

靶向翻译后修饰

unmodified

基因信息

human ... PRDM16(63976)

一般描述

Anti-PRDM16, Cat. No. ABE543, is a rabbit polyclonal antibody that detects PR domain zinc finger protein 16 (PRDM16) and is teste for use in Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

免疫原

KLH-conjugated linear peptide corresponding to human PRDM16.

应用

Detect PRDM16 using this rabbit polyclonal antibody, Anti-PRDM16 Antibody validated for use in western blotting, IP & IHC.
Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated PRDM16 from 500 µg of K562 nuclear extract.

Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected PRDM16 in human kidney tissues.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology

质量

Evaluated by Western Blotting in LNCaP nuclear extract.

Western Blotting Analysis: 2 µg/mL of this antibody detected PRDM16 in 10 µg of LNCaP nuclear extract.

目标描述

~140 kDa observed. Four isoforms are known to exist. This protein has been observed at 150 kDa, 135 kDa, and 100 kDa (EMD Millipore Cat. No. ABD130). Uncharacterized band(s) may be observed in some cell lysates.

外形

Affinity purified
Purified rabbit serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

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Liang-Ru Fei et al.
Journal of experimental & clinical cancer research : CR, 38(1), 35-35 (2019-01-27)
The transcription factor PR domain containing 16 (PRDM16) is known to play a significant role in the determination and function of brown and beige fat. However, the role of PRDM16 in tumor biology has not been well addressed. Here we

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