Anti-Adenovirus 5 Pre-hexon-linking protein IIIa

Pre-hexon-linking protein IIIa (UniProt: P12537; also known as Capsid vertex-specific component IIIa, CVSC, Protein IIIa, pIIIa) is encoded by the L1 gene in Adenovirus. Adenovirus 5 belongs to the Adenoviridae family of non-enveloped viruses that are characterized by linear, double-stranded DNA genomes. Their capsid is an icosahedron that contains at least 12 different viral proteins. Adenovirus 5 is commonly used for gene transfer experiments due to its ability to infect a wide group of different cell types. It can harbor large genes in its genome incorporated via homologous recombination techniques. Pre-hexon-linking protein IIIa is cleaved near the C-terminal region (residues 570-571) by the viral protease during virion maturation to form the mature hexon-linking protein IIIa protein. Maturation primes the viral particle for stepwise uncoating by facilitating penton release and by loosening the condensed genome and its attachment to the icosahedral shell. Protein IIIa that is expressed in the late phase of the viral replicative cycle serves as a structural component of the virion that acts as a cement protein on the capsid exterior and which mediates the interactions between the hexons, including the peripentonal hexons. It also interacts with packaging protein 3 and this interaction is reported to promote correct genome packaging. (Ref.: Charman, M., et al. (2023). Nature. 616(7956):332-338; Pérez-Berná, AJ., et al. (2014). J. Virol. 88(3); 1513-1524; Reddy, VS., and Nemerow, GR. (2014). Proc. Natl. Acad. Sci. USA. 111(32); 11715-11720).

His-tagged, full-length, recombinant Pre-hexon-linking protein IIIa from human Adenovirus 5.

Quality Control Testing

Evaluated by Western Blotting in lysate from HeLa cells infected Human adenovirus type 5.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Adenovirus 5 Pre-hexon-linking protein IIIa in lysate from HeLa cells infected with human adenovirus type 5, but not in lysate from uninfected cells.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Adenovirus 5 Pre-hexon-linking protein IIIa in lysates from Hela cells infected with Human adenovirus type 5.(Courtesy of Dr. Patrick Hearing, Stony Brook University).

Immunofluorescence Analysis: A representative lot detected Adenovirus 5 Pre-hexon-linking protein IIIa in immunofluorescence application. (Charman, M., et al. (2023). Nature. 616(7956):332-338).

Western Blotting Analysis: A representative lot detected Adenovirus Pre-hexon-linking protein IIIa in Western Blotting application. (Subramanian, T., et al. (2006). J. Virol. 80(4); 2000-2012).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

This rabbit polyclonal antibody detects Pre-hexon-linking protein IIIa of Human Adenovirus 5.

Rabbit polyclonal antisera without azide.

Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.