conjugate
unconjugated
Quality Segment
antibody form
affinity purified immunoglobulin
clone
polyclonal
purified by
affinity chromatography
species reactivity
rat, mouse, rabbit
concentration
(Please refer to lot specific datasheet.)
technique(s)
immunohistochemistry: suitable, western blot: suitable
UniProt accession no.
target post-translational modification
unmodified
General description
Linear peptide corresponding to mouse Stearoyl-CoA desaturase 1.
~30 kDa observed
Application
Western Blotting Analysis: A representative lot detected Stearoyl-CoA desaturase 1 in microsomal membranes from C57BL/6J mice (Thomas Gettys, Palmetto Biotech).
Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected Stearoyl-CoA desaturase 1 in brown and white adipose mouse hepatic tissue sections (Thomas Gettys, Palmetto Biotech).
Western Blotting Analysis: A representative lot detected Stearoyl-CoA desaturase 1 in mouse microsomal liver preps (Hasek, B.E., et al. (2013). Diabetes. 62:1-12).
Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected Stearoyl-CoA desaturase 1 in brown and white adipose mouse hepatic tissue sections (Thomas Gettys, Palmetto Biotech).
Western Blotting Analysis: A representative lot detected Stearoyl-CoA desaturase 1 in mouse microsomal liver preps (Hasek, B.E., et al. (2013). Diabetes. 62:1-12).
Physical form
Purified rabbit polyclonal in buffer containing PBS without preservatives.
Preparation Note
Stable for 1 year at -80°C from date of receipt.Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -80°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Evaluated by Western Blotting in microsomal preparation of rat brain tissue lysate.
Western Blotting Analysis: A 1:100 dilution of this antibody detected Stearoyl-CoA desaturase 1 in 10 µg of microsomal preparation of rat brain tissue lysate.
Western Blotting Analysis: A 1:100 dilution of this antibody detected Stearoyl-CoA desaturase 1 in 10 µg of microsomal preparation of rat brain tissue lysate.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.