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Merck
CN

ABN1381

抗-磷酸-TrkB（Tyr816）抗体

from rabbit, purified by affinity chromatography

别名:

BDNF/NT-3 growth factors receptor, Neurotrophic tyrosine kinase receptor type 2, TrkB tyrosine kinase, Trk-B, phospho-TrkB(Tyr816)

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

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产品名称

抗-磷酸-TrkB（Tyr816）抗体, from rabbit, purified by affinity chromatography

biological source

rabbit

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, rat

species reactivity (predicted by homology)

gorilla (based on 100% sequence homology), bovine (based on 100% sequence homology), porcine (based on 100% sequence homology), human (based on 100% sequence homology), horse (based on 100% sequence homology)

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

NCBI accession no.

NP_036863

UniProt accession no.

Q63604

shipped in

wet ice

target post-translational modification

phosphorylation (pTyr816 )

Quality Level

100

Gene Information

human ... NTRK2(4915)
rat ... Ntrk2(25054)

相关类别

Analysis Note

通过蛋白质印迹在BDNF处理的鼠TrkB转染的HEK293细胞裂解物中进行了评估。

蛋白质印迹分析：该抗体的1：500稀释液在10 µg BDNF处理的鼠TrkB转染的HEK293细胞裂解物中检测到磷酸化TrkB（Tyr816）。

Application

使用经验证可用于蛋白质印迹、免疫细胞化学、免疫沉淀和免疫组化的抗磷酸化TrkB（Tyr816）抗体检测磷酸化-TrkB（Tyr816）。
免疫细胞化学分析：一个代表性批次在培养的海马神经元中检测到BDNF和腺苷刺激的TrkB Tyr816磷酸化（由Dr. Moses V. Chao, Langone Medical Center, New York University提供）。
免疫沉淀分析：代表性批次免疫沉淀来自表达转染TrkB的BDNF刺激的HEK293细胞的Tyr816磷酸化TrkB（由Dr. Moses V. Chao, Langone Medical Center, New York University提供）。
蛋白质印迹分析：代表性批次在产后P9–P10大鼠的皮质切片以及腹膜内注射Dex注射的P18-20大鼠的背侧纹状体中检测到地塞米松-（Dex-）和BDNF诱导的TrkB Tyr816磷酸化（Jeanneteau, F., et al. (2008).Proc Natl Acad Sci USA.105(12):4862-4867）。
蛋白质印迹分析：代表性批次在小鼠脑突触制备物中检测到TrkB Tyr816磷酸化（C.N., et al. (2013).Cell.155(7):1596-1609）。
蛋白质印迹分析：代表性批次在培养的小鼠海马神经元和表达鼠TrkB的HEK293细胞中检测到BDNF刺激的TrkB Tyr816磷酸化（Bath, K.G., et al. (2008).J. Neurosci. 28(10):2383-2393）。
免疫组织化学分析：代表性批次在接受甲吡酮和/或地塞米松治疗后的产后P18大鼠的齿状回组织切片中检测到增强的TrkB Tyr816磷酸化（Jeanneteau, F., et al. (2008).Proc Natl Acad Sci USA.105(12):4862-4867）。
免疫组化分析：代表性批次在来自成年小鼠的嘴侧迁移流（RMS）的冠状切片中检测到TrkB Tyr816磷酸化（ath, K.G., et al. (2008).J. Neurosci. 28(10):2383-2393）。
免疫细胞化学分析：代表性批次在5小时饥饿的大鼠海马神经元培养中检测到皮质酮刺激的TrkB Tyr816磷酸化（Jeanneteau, F., et al. (2008).Proc Natl Acad Sci USA.105（12）：4862-4867）。
免疫细胞化学分析：代表性批次在来自E16.5大鼠胚胎的培养DRG神经元中检测到BDNF刺激的TrkB Tyr816磷酸化（Arevalo, J.C., et al. (2006).Neuron.50(4):549-559）。
研究子类别
发育信号转导
研究类别
神经科学

Biochem/physiol Actions

该多克隆抗体选择性检测BDNF诱导的原代胚胎大鼠E18海马神经元中糖基化（145 kDa）和未加工（110 kDa）TrkB的pTyr816磷酸化，但不检测NGF诱导的原代胚胎间隔神经元（PMID 18347336）中TrkA的pTyr490或pTyr794磷酸化。

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General description

BDNF/NT-3生长因子受体（EC 2.7.10.1；UniProt Q63604；也称为GP145-TrkB/GP95-TrkB，Trk-B，TrkB酪氨酸激酶，神经营养因子酪氨酸受体激酶2型，神经受体蛋白酪氨酸激酶）由大鼠中的Ntrk2基因（也称为RATTRKB1，Trkb，TRKB1）编码（基因ID 25054）。神经营养因子通过两类跨膜受体Trk受体酪氨酸激酶和p75神经营养因子受体介导广泛的神经元功能，包括存活，分化，生长和死亡。虽然神经生长因子（NGF），脑源性神经营养因子（BDNF），神经营养因子3（NT-3）和NT-4全部结合p75受体，但TrkA对NGF具有选择性，TrkB对BDNF和NT-4具有选择性，而TrkC对NT-3具有选择性。TrkA和TrkB受体也可以被G蛋白偶联受体（GPCR）的配体激活，包括核苷腺苷或腺苷激动剂，例如CGS 21680和垂体腺苷酸环化酶激活多肽（PACAP）。除了通过GPCR进行反式激活外，据报告，由于Trk和GC受体（GR）之间的信号串扰，糖皮质激素还可以促进TrkA/B/C激活。
观察值〜140 kDa

Immunogen

对应于C末端附近具有磷酸化Tyr816的大鼠TrkB序列的线性肽。
表位：近C端

Other Notes

浓度：请参考特定批次的数据表。

Physical form

亲和纯化
纯化的兔多克隆抗体，溶于含有0.1 M Tris-甘氨酸（pH 7.4）、150 mM NaCl和0.05％叠氮化钠的缓冲液中。

Preparation Note

自收到之日起，在 2-8°C 条件下可稳定保存1年

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存储类别

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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