ABN1382
Anti-DGL-α Antibody
from rabbit, purified by affinity chromatography
别名:
Sn1-specific diacylglycerol lipase alpha, DGL-alpha, Neural stem cell-derived dendrite regulator, DGL-α
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
immunohistochemistry
western blot
western blot
Species reactivity:
mouse, human
Citations:
1
Technique(s):
immunohistochemistry: suitable
western blot: suitable
western blot: suitable
Uniprot accession no.:
产品名称
Anti-DGL-α Antibody, from rabbit, purified by affinity chromatography
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse, human
technique(s)
immunohistochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... DAGLA(747)
Analysis Note
Evaluated by Western Blotting in mouse cerebellum tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected DGL-α in 10 µg of mouse cerebellum tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected DGL-α in 10 µg of mouse cerebellum tissue lysate.
Application
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected DGL-α in human cerebellum and mouse cerebellum tissue.
Immunohistochemistry Analysis: A representative lot detected DGL-α in mouse brain tissue (Berghuis, P., et al. (2007). Science. 316:1212-1216).
Immunohistochemistry Analysis: A representative lot detected DGL-α in mouse brain tissue (Berghuis, P., et al. (2007). Science. 316:1212-1216).
This Anti-DGL-α Antibody is validated for use in Western Blotting and Immunohistochemistry for the detection of DGL-α.
General description
The protein DGL-alpha is a lipase enzyme that catalyzes the hydrolysis of diacylglycerol (DAG) to 2-arachidonoyl-glycerol (2-AG) endocannabinoid in tissues. Endocannabinoids are a group of lipid-like molecules that are neuromodulatory in nature and are involved in the control of many physiological functions including appetite, pain sensation, mood, and memory. They also mediate the psychoactive effects of cannabis (marijuana). Endocannabinoids (ECs) act as retrograde messengers that enable postsynaptic cells to regulate the strength of their synaptic inputs. DGL-alpha is also an excellent marker for cerebellum as DGL-alpha is highly expressed in cerebellum and necessary for the regulation of synaptic retrograde transmission in the cerebellum, hippocampus and striatum, as well as other regions including the auditory brainstem.
~110 kDa observed. Uncharacterized band(s) may appear in some lysates.
Immunogen
Recombinant protein corresponding to the cytoplasmic domain of human DGL-α.
Other Notes
Concentration: Please refer to lot specific datasheet.
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
B A S Reyes et al.
Neuroscience, 303, 323-337 (2015-07-15)
Endocannabinoids (eCBs) are involved in a myriad of physiological processes that are mediated through the activation of cannabinoid receptors, which are ubiquitously distributed within the nervous system. One neurochemical target at which cannabinoids interact to have global effects on behavior
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