Anti-Rootletin

Rootletin (UniProt: Q8CJ40; also known as Ciliary rootlet coiled-coil protein) is encoded by the Crocc (also known as Kiaa0445) gene (Gene ID: 230872) in murine species. Rootletin is a major structural component of the ciliary rootlet, a cytoskeletal-like structure in ciliated cells that originates from the basal body at the proximal end of a cilium and extends proximally toward the cell nucleus. It contributes to centrosome cohesion before mitosis. In ciliated cells, it is associated with ciliary rootlets, but in non-ciliated cells it is localized between, around and at the proximal ends of the centrioles. It dissociates from the centrioles at the onset of D7mitosis and re-associates with them at anaphase. Highest expression of Rootletin is found in photoreceptor cells of retina. Lower expressions have been reported in brain, trachea and kidney. In retinal photoreceptors rootlets extend from the basal bodies to the synaptic terminals and anchor endoplasmic reticulum membranes along their length. During embryonic development, it is enriched along the apical domains of neuro epithelium in brain ventricular zone, in primordia of retinal pigment epithelia, and in neural retina. Rootletin contains a globular head domain and a tail domain that consists of extended coiled-coil structures. The head domain may be required for targeting to the basal body and binding to a kinesin light chain. (Ref.: Yang, J., et al. (2002). J. Cell Biol. 159(3): 431-440).

~227 kDa observed; 226.95 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

His-tagged recombinant fragment corresponding to 188 amino acids from the C-terminal region of murine Rootletin.

Anti-Rootletin Antibody, Cat. No. ABN1714, is a highly specific rabbit polyclonal antibody that targets Rootletin and has been tested in Immunocytochemistry, Immunohistochemistry (Paraffin), and Immunoprecipitation.

Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected Rootletin in mouse cerebral cortex and mouse retina tissues.

Immunocytochemistry Analysis: A representative lot detected Rootletin in Immunocytochemistry applications (Veleri, S., et. al. (2014). Nat Commun. 5:4207).

Immunoprecipitation Analysis: A representative lot detected Rootletin in Immunoprecipitation applications (Yang, J., et. al. (2002). J Cell Biol. 159(3):431-40).

Immunofluorescence Analysis: A representative lot detected Rootletin in Immunofluorescence applications (Sun, X., et. al. (2012). Cilia. 1(1):21).

Research Category
Neuroscience

This rabbit polyclonal antibody detects Rootletin in murine hippocampus and cerebral cortex. It targets an epitope within 188 amino acids from the C-terminal region.

Purified rabbit polyclonal antibody in PBS with 50% glycerol and without azide.

Affinity Purified

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Evaluated by Immunohistochemistry in mouse hippocampus.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Rootletin in mouse hippocampus.

Concentration: Please refer to lot specific datasheet.

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