Anti-phospho NFkB p52, (Ser222) Antibody

Evaluated by Western Blotting in cell lysate U2OS transfected with p52 WT.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected phospho NFkB p52, (Ser222) in 10 µg of cell lysate U2OS transfected with p52 WT.

Anti-phospho NFkB p52, (Ser222) is an antibody against phospho NFkB p52 for use in Western Blotting.

Research Category
Signaling

Research Sub Category
Signaling Neuroscience

Western Blotting Analysis: A representative lot detected phospho NFkB p52, (Ser222) in CD40L and NTL cell lysate (Neil D. Perkins, Newcastle University).

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The p52 NF-kB subunit is induced through activation of the alternative (noncanonical) NF-kB pathway in response to a range of stimuli such as lymphotoxin-b, BAFF and CD40, as well as DNA damage. The p52 formation results from proteolytic processing of its precursor protein p100 as a consequence of IKKalpha-mediated phosphorylation. The p52 is most commonly found as a heterodimer with RelB, although other dimer combinations have been identified by ChIP analysis. Ser222 of NF-kappa B p52 is phosphorylated by the kinase GSK3beta. Phosphorylation at S222 appears to regulate not only p52 partner dimerization but also the p52 dependent activation or repression of Skp2 gene expression.

~52 kDa observed. Uncharacterized band(s) may appear in some lysates.

Epitope: RHD domain

KLH-conjugated linear peptide corresponding to the RHD domain of human phospho NFkB p52, (Ser222).

Concentration: Please refer to lot specific datasheet.

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.